Evidence map›Paper›PMID 40234586›Full record

ArticleScientific reports2025

Polystyrene microplastics exposure in freshwater fish, Labeo rohita: evaluation of physiology and histopathology.

Eram Rashid, Syed Makhdoom Hussain, Shafaqat Ali, Dariusz Kucharczyk, Joanna Nowosad, Khalid A Al-Ghanim

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Eram RashidFish Nutrition Laboratory, Department of Zoology, Government College University Faisalabad, Faisalabad, Pakistan.
Syed Makhdoom HussainFish Nutrition Laboratory, Department of Zoology, Government College University Faisalabad, Faisalabad, Pakistan. drmakhdoomhussain@gcuf.edu.pk.
Shafaqat AliDepartment of Environmental Sciences, Government College University, Faisalabad, 38000, Punjab, Pakistan. shafaqataligill@gcuf.edu.pk.
Dariusz KucharczykDepartment of Research and Development, Chemprof, Gutkowo 54B, Olsztyn, 11-041, Poland.
Joanna NowosadDepartment of Research and Development, Chemprof, Gutkowo 54B, Olsztyn, 11-041, Poland.
Khalid A Al-GhanimDepartment of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The goal of this investigation was to assess the adverse impacts of polystyrene microplastics (PS-MPs) on Labeo rohita with a comprehensive assessment of growth performance, hematological changes, and histopathological effects. Six test diets were established with different MPs levels using canola meal as the basal diet: control 0.0%, 0.5%, 1%, 1.5%, 2%, and 2.5% MPs. For 90 days, 315 fish (15 fish per tank with three replicates) were fed experimental diets at a rate of 5% of their live, twice a day. L. rohita fingerlings treated with 2.5% MPs leading to a notable decline in growth and feed consumption (P < 0.05). As PS-MPs increased, carcass, mineral and digestibility content declined. Additionally, a histology of the gut revealed significant abnormalities in intestine, including villi structure disruption and increased mucus cell proliferation, at a 2.5% PS-MPs concentration. Hematological indices such as RBCs, Hb, PLT, MCHC, and PCV decreased significantly when exposed to MPs, whereas WBCs, MCH, and MCV dramatically increased. Conclusively, this study demonstrated that the PS-MPs exert detrimental effects on growth performance, and induce histopathological and hematological changes in L. rohita.

Indexed as

CyprinidaeMicroplasticsPolystyrenesWater Pollutants, ChemicalAnimalsFresh WaterMicroplasticsPolystyrenesWater Pollutants, ChemicalBody compositionCanola mealLabeo rohitaMicroplasticMinerals composition

Identifiers

PMID40234586
PMCPMC12000475

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.