Evidence map›Paper›PMID 40231909›Full record

ArticleFoods (Basel, Switzerland)2025

Development of Visual Loop-Mediated Isothermal Amplification Assays for Foodborne Hepatitis A Virus.

Tongcan An, Mengyuan Song, Xiang Li, Yingjie Pan, Yong Zhao, Haiquan Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Foods (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tongcan AnCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Mengyuan SongCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Xiang LiTechnical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai 201315, China.
Yingjie PanCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Yong ZhaoCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Haiquan LiuCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Funding

" Science and Technology Innovation Action Plan" Agricultural Field Project 22N31900600
6 · The paper itself

Abstract

(1) Background: There are many cases of human disease caused by the hepatitis A virus contamination of aquatic products, so the development of the rapid detection of hepatitis A virus in aquatic products is crucial. (2) Methods: In this study, we developed three visual loop-mediated isothermal amplification methods for the rapid and intuitive detection of hepatitis A virus in aquatic products. New specific LAMP primers were designed for the HAV-specific VP1 protein shell. (1) HNB dye was added to the LAMP reaction system. After the reaction, the color of the reaction mixture changed from violet to sky blue, showing a positive result. (2) Cresol red dye was added to the LAMP reaction system, and a positive result was indicated by orange, while a negative result was indicated by purple. (3) By labeling FIP with biotin and LF with 6-FAM, the amplified product simultaneously contained biotin and 6-FAM, which bound to the anti-biotin antibody on the gold nanoparticles on the lateral flow dipstick (LFD). Subsequently, biotin was further combined with the anti-fam antibody on the T-line of the test strip to form a positive test result. (3) Results: The three visual LAMP methods were highly specific for HAV. The sensitivity of the visual assay was 2.59 × 10

Indexed as

colorimetric methodhepatitis A viruslateral flow dipstickvisualization detection

Identifiers

PMID40231909
PMCPMC11941577

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.