Evidence map›Paper›PMID 40231109›Full record

ArticleRevista cientifica odontologica (Universidad Cientifica del Sur)

Primary cell culture and characterization of a pleomorphic adenoma. A case report.

Selenne Romero-Servin, Nancy Leticia Mendoza-Martínez, Francisco Germán Villanueva-Sánchez, Ilse Patricia Rodríguez-Tapia, Aylin Divina Cadena-Galeana, René García-Contreras

Abstract readCase Reports
In one paragraph

Article in Revista cientifica odontologica (Universidad Cientifica del Sur). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Selenne Romero-ServinInterdisciplinary Research Laboratory (LII), Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon, National Autonomous University of Mexico. Leon, Mexico. selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com Universidad Nacional Autónoma de México Interdisciplinary Research Laboratory (LII) Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon National Autonomous University of Mexico Leon Mexico selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com.ORCID https://orcid.org/0000-0002-4992-0567
Nancy Leticia Mendoza-MartínezInterdisciplinary Research Laboratory (LII), Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon, National Autonomous University of Mexico. Leon, Mexico. selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com Universidad Nacional Autónoma de México Interdisciplinary Research Laboratory (LII) Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon National Autonomous University of Mexico Leon Mexico selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com.ORCID https://orcid.org/0000-0002-8827-0827
Francisco Germán Villanueva-SánchezInterdisciplinary Research Laboratory (LII), Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon, National Autonomous University of Mexico. Leon, Mexico. selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com Universidad Nacional Autónoma de México Interdisciplinary Research Laboratory (LII) Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon National Autonomous University of Mexico Leon Mexico selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com.ORCID https://orcid.org/0000-0003-3567-5393
Ilse Patricia Rodríguez-TapiaInterdisciplinary Research Laboratory (LII), Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon, National Autonomous University of Mexico. Leon, Mexico. selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com Universidad Nacional Autónoma de México Interdisciplinary Research Laboratory (LII) Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon National Autonomous University of Mexico Leon Mexico selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com.
Aylin Divina Cadena-GaleanaInterdisciplinary Research Laboratory (LII), Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon, National Autonomous University of Mexico. Leon, Mexico. selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com Universidad Nacional Autónoma de México Interdisciplinary Research Laboratory (LII) Oral and Maxillofacial Pathology area, National School of Higher Studies (ENES) Leon National Autonomous University of Mexico Leon Mexico selenneservin@gmail.com nancy.camille18@gmail.com fgvillanuevas@enes.unam.mx ilsertapia@gmail.com aylincadenag@gmail.com.ORCID https://orcid.org/0009-0009-8910-2524
René García-ContrerasInterdisciplinary Research Laboratory (LII); Nanostructures and Biomaterials area, National School of Higher Studies (ENES) Leon, National Autonomous University of Mexico. leon, Mexico. rgarciac@enes.unam.mx Universidad Nacional Autónoma de México Interdisciplinary Research Laboratory (LII) Nanostructures and Biomaterials area, National School of Higher Studies (ENES) Leon National Autonomous University of Mexico leon Mexico rgarciac@enes.unam.mx.ORCID https://orcid.org/0000-0003-3504-5519

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: This case report aimed to characterize primary pleomorphic adenoma cells obtained from a parotid gland tumor through tissue biopsy, primary cell culture, and immunohistochemical analysis. Methods: A tissue biopsy sample from a 58-year-old patient with pleomorphic adenoma underwent histopathological examination and primary cell culture. The primary cells were characterized through immunohistochemical staining using antibodies against S-100, SMA, Vimentin, and cytokeratin AE1/AE3. Additionally, paraffin-embedded tissue sections were stained using cytokeratin AE1/AE3, Vimentin, Calponin, SMA, S100, and p63. Results: Primary cell culture revealed weak S-100 staining and positive SMA in myoepithelial cells, while Vimentin and AE1/AE3 were negative in all the cell population. In the paraffin-embedded tissue, cytokeratin exhibited strong cytoplasmic and membranous positivity in luminal cells. Vimentin showed cytoplasmic staining in myoepithelial cells. S-100 displayed weak nuclear and strong cytoplasmic immunoreactivity in myoepithelial cells. SMA presented weak membranous positivity in a few myoepithelial cells, and finally, p63 showed nuclear staining in abluminal cells. Calponin showed negative staining in neoplastic cells and stroma. Conclusion: The results showed that primary component of pleomorphic adenoma comprises myoepithelial cells. The identification of cell cultured

Indexed as

immunomarkerspleomorphic adenomaprimary cell culturesalivary glandssalivary gland tumors

Identifiers

PMID40231109
PMCPMC11994098

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