ArticleAnnals of botany2025
Modulation of calcium, callose synthesis, membrane permeability and pectin methyl-esterase activity affect cell wall composition and embryo yield during Brassica napus microspore embryogenesis.
Article in Annals of botany, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Integrated transcriptome and proteome indicate molecular mechanisms of endogenous hormonal imbalance regulating variation in jujube (Food chemistry. Molecular sciences · 2026Article
- Unresolved ER stress restricts in vitro plant cell totipotency.Plant cell reports · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
BACKGROUND AND
aimsMicrospore embryogenesis is a convenient inducible system to study the changes associated with the developmental reprogramming of cells. In this work, Brassica napus microspore cultures were used to study the role in the embryogenic switch of callose and pectin cell wall composition, which depends on Ca2+ levels.
methodsWe used different chemicals to modulate Ca2+, callose and pectin methyl-esterification, including Ca(NO3)2, inositol 1,4,5-trisphosphate, 2-deoxy-d-glucose, benzyl alcohol, chitosan, epigallocatechin gallate and pectin methyl-esterase. Ca2+ distribution, callose and cellulose were imaged with FluoForte, Aniline Blue and Pontamine Fast Scarlet stainings, respectively, and observed with confocal microscopy. KEY
resultsInhibition of callose synthesis with 2-deoxy-d-glucose demonstrated that callose is essential for induction of microspore embryogenesis. A moderate increase of Ca2+ levels with Ca(NO3)2 or inositol 1,4,5-trisphosphate promoted increased callose synthesis and deposition in the cell wall. However, the use of benzyl alcohol and chitosan to permeabilize the plasma membrane and allow for Ca2+ influx was not positive, because this prevented embryo development by inducing callus formation. Benzyl alcohol did not affect callose and cellulose deposition, but chitosan induced the formation of callose plugs, similar to those formed in response to pathogen attack. Inhibition of pectin methyl-esterase activity with epigallocatechin gallate during the first 3 days of culture produced ~70 % more embryos, but prolonged exposures were negative. Instead, increased pectin methyl-esterase activity during the first 3 days was not positive, but when applied for 7 days, embryos increased by ~60 %.
conclusionsTogether, these results confirm the relevant role of calcium and callose during the first stages of microspore induction and suggest that the levels of pectin methyl-esterification in the cell wall are dynamic and that different cell wall compositions are required during the different stages of microspore embryogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.