Evidence map›Paper›PMID 40229797›Full record

ArticleMicrobial cell factories2025

A novel IgG-Fc-Fused multiepitope vaccine against Brucella: robust immunogenicity.

Aodi Wu, Yuting Zhang, Caidong Liu, Kaiat Zhumanov, Tao He, Kexin Yan, Honghuan Li, Shuangshaung Fu, Xin Li, Wenxiang Zhang and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Aodi Wu *College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Yuting Zhang *College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Caidong LiuSchool of Medicine, Shihezi University, Shihezi, 832003, Xinjiang, China.
Kaiat ZhumanovKazakh National Agrarian University, 050010, Almaty, Kazakhstan.
Tao HeCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Kexin YanCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Honghuan LiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Shuangshaung FuCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Xin LiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Wenxiang ZhangCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Chuang MengJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China.
Changsuo ZhangTiankang Biopharmaceutical Co., Ltd, 830032, Urumqi, Xinjiang, China.
Jinliang ShengCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Zhongchen MaCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Mingguo XuCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Junbo ZhangGuizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren University, Tongren, Guizhou, 554300, China. zhangjunbo666@126.com.
Jihai YiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China. 724050645@qq.com.
Yueli WangSchool of Medicine, Shihezi University, Shihezi, 832003, Xinjiang, China. 577674101@qq.com.

Funding

Eighth Division Shihezi Science and Technology Plan Project 2024SF01National Natural Science Foundation of China 82160391Open Project of Key Laboratory of Animal Biopharmaceutical Corps FM0F2024001Open Project Program of Jiangsu Key Laboratory of Zoonosis R2104Shihezi University International Science and Technology Cooperation Promotion Program GJHZ202203Tongren Science and Technology Bureau Project TSKY [2023] 39, TSKY [2024] 6Youth Outstanding Science and Technology Talent Project of Department of Education of Guizhou Province QJJ [2024] 347
6 · The paper itself

Abstract

Brucellosis is one of the most common zoonotic diseases caused by Brucella spp. However, there is currently no Brucella vaccine available for humans. Although some attenuated live vaccines have been approved for animals, their protective efficacy is suboptimal. In previous studies, we utilized an epitope- and structure-based vaccinology platform to identify the immunodominant epitopes of Brucella antigens OMP19, OMP16, OMP25, and L7/L12, and constructed the multi-epitope vaccine MEV-Fc against Brucella. In this study, OMP19, OMP16, OMP25, and L7/L12, and MEV-Fc was expressed and purified via an Escherichia coli expression system, which validated that MEV-Fc possesses high immunological efficacy and exerts a significant protective effect in BALB/c mice within the Brucella infection model. MEV-Fc enhanced Th1 and Th2 immune responses and strongly induced the production of the pro-inflammatory cytokine IFN-γ. Furthermore, MEV-Fc protected mice against Brucella infection compared to control group (PBS). In conclusion, our results provide new insights and data support for the development of human Brucella vaccines.

Indexed as

BrucellaBrucella VaccineBrucellosisEpitopesImmunoglobulin GAnimalsAntigens, BacterialFemaleInterferon-gammaMiceMice, Inbred BALB CAntigens, BacterialBrucella VaccineEpitopesImmunoglobulin GInterferon-gammaBrucellaMultiple epitopesVaccines immune response

Identifiers

PMID40229797
PMCPMC11998165

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.