Evidence map›Paper›PMID 40229791›Full record

ArticleJournal of orthopaedic surgery and research2025

Quercetin-primed MSC exosomes synergistically attenuate osteoarthritis progression.

Mingfeng Lu, Aiju Lou, Junqing Gao, Shilin Li, Lilei He, Weifeng Fan, Lilian Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mingfeng Lu *The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine, Foshan, Guangdong, 528000, China.
Aiju Lou *Department of Rheumatology, Liwan Central Hospital of Guangzhou, Guangzhou, Guangdong, 510030, China.
Junqing GaoThe Eighth Clinical Medical College of Guangzhou University of Chinese Medicine, Foshan, Guangdong, 528000, China.
Shilin LiThe Eighth Clinical Medical College of Guangzhou University of Chinese Medicine, Foshan, Guangdong, 528000, China.
Lilei HeThe Eighth Clinical Medical College of Guangzhou University of Chinese Medicine, Foshan, Guangdong, 528000, China.
Weifeng FanThe Eighth Clinical Medical College of Guangzhou University of Chinese Medicine, Foshan, Guangdong, 528000, China. 13106664870@163.com.
Lilian ZhaoThe Eighth Clinical Medical College of Guangzhou University of Chinese Medicine, Foshan, Guangdong, 528000, China. zhaolilian@163.com.

Funding

Guangdong Province Medical Science and Technology Research Foundation Project A2024661
6 · The paper itself

Abstract

backgroundOsteoarthritis (OA), a degenerative joint disease characterized by cartilage degradation and inflammation, lacks effective disease-modifying therapies. Quercetin, a bioactive flavonoid derived from Traditional Chinese Medicine, exhibits anti-inflammatory and chondroprotective properties but is limited by poor bioavailability. Mesenchymal stem cell-derived exosomes (MSC-Exos) offer a promising strategy for targeted drug delivery and cartilage regeneration.

methodsBone marrow-derived MSC exosomes (Que-Exo) were isolated after preconditioning with quercetin (1µM, 24 h). Their effects were evaluated in IL-1β-stimulated chondrocytes via RT-qPCR, Western blot, transcriptomics, and proteomics. An ACLT-induced OA mouse model received intra-articular injections of Que-Exo, with cartilage integrity assessed by Safranin O staining and OARSI scoring.

resultsQue-Exo significantly reduced IL-1β-induced pro-inflammatory markers (MMP9 and COX-2) and restored cartilage repair genes (SOX9 and Collagen II) compared to untreated exosomes. Multi-omics analyses revealed activation of PI3K-AKT signaling and glutathione metabolism pathways. In vivo, Que-Exo mitigated cartilage degradation and preserved proteoglycan content.

conclusionsQuercetin-preconditioned MSC exosomes synergistically enhance chondroprotection and anti-inflammatory effects, offering a novel therapeutic strategy for OA by combining herbal bioactive compounds with exosome-mediated delivery.

Indexed as

Disease ProgressionExosomesMesenchymal Stem CellsOsteoarthritisQuercetinAnimalsChondrocytesDisease Models, AnimalMaleMiceMice, Inbred C57BLQuercetinChondroprotectionEngineered exosomesMulti-omics analysisOsteoarthritisQuercetin

Identifiers

PMID40229791
PMCPMC11998445

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.