Evidence map›Paper›PMID 40227556›Full record

ArticleJournal of neuro-oncology2025

Glioma-derived SPARCL1 promotes the formation of peritumoral neuron-glioma synapses.

Yang Li, Yao Wang, Xingyue Han, Jing Xu, Enyu Liu, Jingmin Cheng, Yuan Ma, Tao Yang, Jianping Wu, Haodong Sun and 6 more

Erratum issuedAbstract read
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In one paragraph

Article in Journal of neuro-oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Yang Li *Department of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Yao Wang *Department of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Xingyue HanDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Jing XuDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Enyu LiuDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Jingmin ChengDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Yuan MaDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Tao YangDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Jianping WuDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Haodong SunDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Kexia FanDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Danyi ShenDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Jie LiDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Xin ChenDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Sixun YuDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
Haifeng ShuDepartment of Neurosurgery, The General Hospital of Western Theater Command, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China. shuhaifeng@swjtu.edu.cn.ORCID http://orcid.org/0000-0002-0193-1881

Funding

Joint key project of the General Hospital of the Western Theater Command of China [2019LH01]Medical Innovation Project [21WQ040]Project of Technology Innovation Talent Project of Sichuan Provincial Department of Science and Technology [2022JDRC0041]
6 · The paper itself

Abstract

purposeGliomas are the most common type of primary malignant brain tumor with high degree of malignancy and rapid progression, and patients often have complications such as epilepsy and cognitive impairment. Thus, identifying related therapeutic targets, prolonging patient survival time and improving patient quality of life are urgently needed. Recent studies have shown that glutamatergic neurons around tumors and glioma cells form synapses, neuron-glioma synapses (NGSs), which have electrophysiological properties and participate in the proliferation, infiltration and invasion of tumors. Therefore, we aimed to explore the molecular mechanisms underlying NGS formation.

methodsWe used bioinformatic analysis to screen for the expression of SPARCL1, which may play a role in promoting NGS formation, and we evaluated clinical samples through immunofluorescence, Western blot, and reverse transcriptase polymerase chain reaction (RT‒PCR) assays to validate the bioinformatic analysis results. Vitro neuron-glioma cell coculture model was established and allowed us to edit SPARCL1 expression in glioma cells, further allowing us to investigate the role of SPARCL1 in NGS formation.

resultsBioinformatic analysis revealed that SPARCL1 is highly expressed in glioma cells and is associated with synaptogenesis. Clinical samples were evaluated to verify the bioinformatics results, and SPARCL1 was found to be highly distributed in the tumor peripheral region. In the vitro neuron-glioma cell coculture model, NGSs were clearly observed, and SPARCL1 overexpression promoted NGS formation.

conclusionTaken together, these findings suggest that SPARCL1 is one of the molecules that promotes NGS formation in the tumor peripheral region.

Indexed as

Brain NeoplasmsCalcium-Binding ProteinsExtracellular Matrix ProteinsGliomaNeuronsSynapsesCell Line, TumorCell ProliferationCoculture TechniquesGene Expression Regulation, NeoplasticHumansMaleCalcium-Binding ProteinsExtracellular Matrix ProteinsSPARCL1 protein, humanGliomaHevinNGSSPARCL1Synaptogenesis

Identifiers

PMID40227556
PMCPMC12170744

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.