Evidence map›Paper›PMID 40227424›Full record

ArticleInternational journal of legal medicine2025

Identification of circular RNAs as biomarkers for pupal age estimation and postmortem interval in forensically important Sarcophaga peregrina (Diptera: Sarcophagidae).

Hai Wu, Haojie Tang, Xing Han, Fernand Jocelin Ngando, Yanjie Shang, Yadong Guo

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Article in International journal of legal medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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0cells of the map it votes in
3citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Hai Wu *Department of Forensic Science, Xiangya School of Basic Medical Sciences, Central South University, Changsha, Hunan, 410013, China.
Haojie Tang *Department of Forensic Science, Xiangya School of Basic Medical Sciences, Central South University, Changsha, Hunan, 410013, China.
Xing HanDepartment of Forensic Science, Xiangya School of Basic Medical Sciences, Central South University, Changsha, Hunan, 410013, China.
Fernand Jocelin NgandoDepartment of Forensic Science, Xiangya School of Basic Medical Sciences, Central South University, Changsha, Hunan, 410013, China.
Yanjie ShangDepartment of Forensic Science, Xiangya School of Basic Medical Sciences, Central South University, Changsha, Hunan, 410013, China. shangyj@csu.edu.cn.
Yadong GuoDepartment of Forensic Science, Xiangya School of Basic Medical Sciences, Central South University, Changsha, Hunan, 410013, China. gdy82@126.com.ORCID http://orcid.org/0000-0001-7468-8061

Funding

the National Natural Science Foundation of China 82402196the Natural Science Foundation of Hunan Province 2024JJ6546
6 · The paper itself

Abstract

Accurate estimation of the pupal age in necrophagous flies is vital for determining the postmortem interval (PMI) in forensic entomology. Differential expression of genes (DEGs) exhibits temporal fluctuations across the developmental stages of these flies. This study evaluates circular RNAs (circRNAs) as novel molecular markers and develops a model for predicting pupal development time based on circRNA expression. Transcriptomic analysis of Sarcophaga peregrina pupae at various stages identified four circRNAs (circRNA_0037, circRNA_0531, circRNA_3373, circRNA_2847) showing significant expression differences. Using real-time quantitative PCR and regression analysis, we constructed a model to estimate development time, which accurately predicts intra-puparial periods. Additionally, we examined circRNA degradation patterns in pupae under lethal conditions and identified a clear degradation trend in circRNA_2847, suggesting its potential use for estimating the PMI. This study introduces new molecular markers and insights for estimating necrophagous fly pupal age.

Indexed as

Postmortem ChangesPupaRNA, CircularSarcophagidaeAnimalsBiomarkersForensic EntomologyGene Expression ProfilingReal-Time Polymerase Chain ReactionBiomarkersRNA, CircularCircular RNAsForensic entomologyIntra-puparial developmentPostmortem intervalSarcophaga peregrina

Identifiers

PMID40227424

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.