ArticleJournal of the American Chemical Society2025
After 75 Years, an Alternative to Edman Degradation: A Mechanistic and Efficiency Study of a Base-Induced Method for N-Terminal Peptide Sequencing.
Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Finding REMO: a sequencing method for recognition-encoded melamine oligomers.Chemical science · 2026Article
- An engineered nanopore identifies saccharides, amino acids, peptides and ribonucleotides.Nature biotechnology · 2026Article
- Exopeptidase-assisted nanopore peptide sequence identification.Nature communications · 2026Article
- Optimization of the Quantum-Si Platinum Single-Molecule Protein Sequencing Platform Toward Improved Complex-Matrix Protein Identification.ACS omega · 2026Article
- Principles ofbioRxiv : the preprint server for biology · 2026Article
- A Graphene Field-Effect Transistor-Based Biosensor Platform for the Electrochemical Profiling of Amino Acids.Biosensors · 2026Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The sequencing of peptides via N-terminal amino acid removal is a classic reaction termed Edman degradation. This method involves repeated treatment of the N-terminal amino group of a peptide with phenyl isothiocyanate (PITC), followed by treatment with trifluoroacetic acid. Spurred by the need for an alternative non-acid-based chemistry for next-generation protein sequencing technologies, we developed a base-induced N-terminal degradation method. Several N-terminal derivatization reagents carrying supernucleophiles were tested. After rounds of iterative designs, compound
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