Evidence map›Paper›PMID 40226421›Full record

ArticleJournal of tissue engineering and regenerative medicine2023

Osteogenesis of Human iPSC-Derived MSCs by PLLA/SF Nanofiber Scaffolds Loaded with Extracellular Matrix.

Junming Zhang, Lingbin Che, Yunliang Wu, Lei Zhou, Li Liu, Yuanhang Yue, Dianwen Song, Xiangxin Lou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of tissue engineering and regenerative medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Junming ZhangCollege of Biological Science and Medical Engineering, Donghua University, Shanghai, China.
Lingbin CheDepartment of Orthopedics, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Yunliang WuCollege of Biological Science and Medical Engineering, Donghua University, Shanghai, China.
Lei ZhouDepartment of Orthopedics, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Li LiuCollege of Biological Science and Medical Engineering, Donghua University, Shanghai, China.
Yuanhang YueCollege of Biological Science and Medical Engineering, Donghua University, Shanghai, China.
Dianwen SongDepartment of Orthopedics, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Xiangxin LouCollege of Biological Science and Medical Engineering, Donghua University, Shanghai, China.ORCID 0000-0002-1673-7715

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone defects that arise from trauma, skeletal diseases, or tumor resections have become the commonest and most thorny problems in orthopedic clinics. Recently, biocomposite materials used as artificial bone repair materials have provided a promising approach for bone regeneration. In this study, poly (l-lactide acid) (PLLA) and silk fibroin (SF) were used to fabricate nanofiber scaffolds by electrospinning technology. In order to simulate a biomimetic osteoblast microenvironment, decellularized extracellular matrix from osteoblasts was loaded into the biocomposite scaffolds (O-ECM/PLLA/SF). It was found that the O-ECM/PLLA/SF scaffolds were nontoxic for L929 cells and had good cytocompatibility. Their effects on mesenchymal stem cells derived from human-induced pluripotent stem cell (iPSC-MSC) behavior were investigated. As a result, the scaffolds with the addition of O-ECM showed enhanced alizarin red S (ARS) activity. In addition, higher expression of osteogenic gene markers such as runt-related transcription factor 2 (Runx2), collagen type I (Col-1), and osteocalcin (OCN) as well as upregulated expression of osteogenic marker protein osteopontin (OPN) and Col-1 further substantiated the applicability of O-ECM/PLLA/SF scaffolds for osteogenesis. Furthermore, the

Indexed as

Extracellular MatrixFibroinsInduced Pluripotent Stem CellsMesenchymal Stem CellsNanofibersOsteogenesisPolyestersTissue ScaffoldsAnimalsCell DifferentiationCell LineGene Expression RegulationHumansMiceRatsRats, Sprague-DawleyFibroinsPolyesterspoly(lactide)

Identifiers

PMID40226421
PMCPMC11918522

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.