ArticleIranian journal of biotechnology2024
Improving Bioplastic Production: Enhanced P(3HB-
Article in Iranian journal of biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
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5 authors.
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Abstract
Background: Biodegradable polyhydroxyalkanoates (PHAs) hold promises for various applications in industries ranging from packaging to biomedical engineering, highlighting the importance of this pioneering research in sustainable materials synthesis. Objectives: The objective of this investigation was to present the successful production of polyhydroxyalkanoate (PHA) copolymer P(3HB- Materials and Methods: To design the inputs conditions,a central composite factorial design (CCFD) based on a one-variable-at-a-time (OVAT) experiment was conducted. This experiment aimed to identify key chemical factors and their operational ranges affecting PHBHHx production by the mutant strain. Later, batch and repeated fed-batch (RFB) culture were run in a stirred tank bioreactor (STBR) with a working volume of 2 L which was inoculated by 200 ml (10% v/v) of freshly grown seed culture (18 h). This methodology ensured controlled exploration of individual variables, facilitating the selection of optimal conditions for PHBHHx production. Total glucose concentrations during fermentation were assessed through the phenol-sulfuric acid assay. Results: The study demonstrates the effectiveness of the designed model in predicting PHBHHx production during fermentation runs with predicted values closely aligning with experimental results. This underscores the model satisfactory fitness with the experimental design. Additionally, a surprising enhancement was observed in the fermentation process with repeated fed-batch (RFB) leading to a substantial increase in cell dry weight (CDW), PHBHHX concentration, and 3HHx fraction, approximately 7 times, 7 times and 4.5 times, respectively. Confirmation of copolymer production was further validated through analytical techniques including FTIR spectroscopy, NMR, and TEM analysis. These findings collectively highlight the promising potential of RFB as a method to significantly improve PHBHHx production covering the way for further advancements in biopolymer manufacturing processes. Conclusions: Our study reveals the potential of newly engineered
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