ArticleIranian journal of biotechnology2024
Arbitrarily Suffixed Sequence-Specific Primer PCR for Reliable Genome Walking: Taking Genome Walkings of
Article in Iranian journal of biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Article
- Bridging PCR-Based Genome-Walking Protocol.Bio-protocol · 2025Article
- Center Degenerated Walking-Primer PCR: A Novel and Universal Genome-Walking Method.Current issues in molecular biology · 2025Article
- Uracil walking primer PCR: An accurate and efficient genome-walking tool.Journal, genetic engineering & biotechnology · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Background: Genome walking has contributed to life science-related areas. Herein, we detailed a new genome walking method, nominated as arbitrarily suffixed sequence-specific primer PCR (ASP-PCR). Objectives: This study aimed to construct an efficient random PCR-based genome walking method. Materials and Methods: The key for this method is the use of a hybrid primer (HP) in primary ASP-PCR. This HP is fabricated by suffixing an arbitrary sequence to outmost sequence-specific primer. The relaxed cycle in primary ASP-PCR facilitates the partial annealing of HP to genome, creating many single-stranded DNAs. In the next stringent cycles, target single-strand is exponentially amplified, because it also has a site complementary to the sequence-specific part of HP; while nontarget cannot be further processed due to lacking such a site. Nested secondary/tertiary ASP-PCR further selectively enriches target DNA. Results: The practicability of ASP-PCR was confirmed by obtaining unknown DNAs adjacent to Conclusions: The ASP-PCR is a promising genome walking scheme, and may have a potential use in life science-related areas.
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