Evidence map›Paper›PMID 40224539›Full record

ArticleBiochemistry and biophysics reports2025

Ultrasound irradiation activates purine metabolism and mitochondrial respiration via the MAPK signaling pathway in myotubes.

Xiaoqi Ma, Noriaki Maeshige, Atomu Yamaguchi, Yunfei Fu, Jihao Xing, Qingcheng Guo, Hao Lin, Fuwen Lu, Hiroyo Kondo, Hidemi Fujino

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In one paragraph

Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Xiaoqi MaDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Noriaki MaeshigeDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Atomu YamaguchiDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Yunfei FuDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Jihao XingDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Qingcheng GuoDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Hao LinDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Fuwen LuShanghai Yangzhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), Tongji University School of Medicine, Shanghai, China.
Hiroyo KondoFaculty of Health and Nutrition, Shubun University, Ichinomiya, Japan.
Hidemi FujinoDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Pulsed ultrasound (US) is widely used both as a diagnostic imaging tool and a therapeutic approach. However, many of the mechanisms underlying the therapeutic effects of non-thermal US remain unclear, especially in skeletal muscles, which play a crucial role in the body's metabolism. The aim of this study was to investigate the effects of US on myotubes. Methods: In this study, C2C12 myoblasts were utilized. After differentiating into myotubes, the cells were exposed to US irradiation at an intensity of 3.0 W/cm Results: Cell viability remained unaffected after US irradiation. The mitogen-activated protein kinase (MAPK) signaling pathway was the most activated, while the expression of various RNAs was significantly altered. Purine metabolism was highly activated, with an increase in the abundance of metabolites associated with this pathway. Furthermore, mitochondrial respiration in the myotubes increased following US irradiation. Conclusion: This study investigated the impact of US irradiation on myotubes using genomic analysis, metabolomic analysis, and mitochondrial function. US irradiation activated the MAPK signaling pathway, which in turn enhanced purine metabolism and improved mitochondrial respiration.

Indexed as

MAPK signaling pathwayMitochondrial respirationMyotubesPulsed ultrasoundPurine metabolism

Identifiers

PMID40224539
PMCPMC11986604

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