ArticleACS omega2025
Single-Step Detection of microRNA via the Programmable DNAzyme Probe-Mediated Multiple Signal Recycling.
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The significant role of microRNAs (miRNAs) in the regulation of pathological ocular neovascularization makes them both biomarkers and therapeutic targets in vascular eye diseases. However, their acute and sensitive detection and regulation continue to be a challenge. An integrative DNA probe (detection probe) was developed to enable the single-step detection of miRNAs with high sensitivity and specificity. This probe combines the functions of specific target recognition, the DNAzyme unit-based signal reaction, and the DNA polymerase/endonuclease-assisted signal cycle. In particular, a single-stranded sequence that engages with the DNAzyme unit of the s2 sequence can identify the target miRNA and liberate the DNAzyme unit to facilitate the signal reaction with the help of metal ions. In addition, the target recycling process and signal cycle were initiated by the DNA polymerase/endonuclease-assisted chain extension and displacement process, resulting in a low limit of detection of 4.56 fM. In addition, the method demonstrated a high level of septicity for the detection of target miRNAs and was capable of distinguishing interfering miRNAs with a one-base mismatch. Our research has shown that the integrative DNAzyme nanomachine is a promising biosensor for the sensitive detection and regulation of miRNA in a single step. It is anticipated that this technology will be used in the early diagnosis and therapy of vascular eye diseases.
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Registered trials
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