Evidence map›Paper›PMID 40223399›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2025

Intravasation-On-µDevice (INVADE): Engineering Dynamic Vascular Interfaces to Study Cancer Cell Intravasation.

Fengtao Jiang, Yingqi Zhang, Guocheng Fang, Yao Wang, Alexander Dupuy, Jasmine Jin, Yi Shen, Khoon S Lim, Yinyan Wang, Yu Shrike Zhang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Microengineered Gradient Hydrogels for Mechanobiology.Advanced healthcare materials · 2026
    Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Fengtao JiangSchool of Biomedical Engineering, Faculty of Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.
Yingqi ZhangSchool of Biomedical Engineering, Faculty of Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.
Guocheng FangSchool of Electrical & Electronic Engineering Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
Yao WangSchool of Biomedical Engineering, Faculty of Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.
Alexander DupuySchool of Biomedical Engineering, Faculty of Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.
Jasmine JinSchool of Biomedical Engineering, Faculty of Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.
Yi ShenSchool of Chemical and Biomolecular Engineering, Faculty of Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.
Khoon S LimCharles Perkins Centre, The University of Sydney, Camperdown, NSW, 2006, Australia.
Yinyan WangSchool of Biomedical Engineering, Faculty of Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.
Yu Shrike ZhangDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.ORCID https://orcid.org/0000-0002-0045-0808
Ann-Na ChoSchool of Biomedical Engineering, Faculty of Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.
Hongxu LuState Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, P. R. China.
Lining Arnold JuSchool of Biomedical Engineering, Faculty of Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.ORCID https://orcid.org/0000-0002-7591-0864

Funding

Australian Research Council DP240101768Australian Research Council DP240102315Australian Research Council FT230100249MRFF Cardiovascular Health Mission Grants MRF2023977National Heart Foundation Vanguard 106979NSW Cardiovascular Capacity Building Program H22/98586Snow Medical Research Foundation 2022SF176the International Partnership Program of Chinese Academy of Sciences 030GJHZ2023098FNthe International Partnership Program of Chinese Academy of Sciences 030GJHZ2024103MITour de Cure Pioneering Research 391-FY2023Wellcome Trust 105863
6 · The paper itself

Abstract

Cancer metastasis begins with intravasation, where cancer cells enter blood vessels through complex interactions with the endothelial barrier. Understanding this process remains challenging due to the lack of physiologically relevant models. Here, INVADE (Intravasation-on-µDevice), a biomimetic microfluidic platform, is presented, enabling high-throughput analysis of cancer cell intravasation under controlled conditions. This engineered platform integrates 23 parallel niche chambers with an endothelialized channel, providing both precise microenvironmental control and optical accessibility for real-time visualization. Using this platform, distinct intravasation mechanisms are uncovered: MCF-7 cells exhibit collective invasion, while MDA-MB-231 cells demonstrate an interactive mode with three functionally distinct subpopulations. A previously unknown epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) switch is We discovered during intravasation, where MDA-MB-231 cells initially increase Vimentin expression before undergoing a 2.3 fold decrease over 96 h alongside a 1.5 fold increase in epithelial cell adhesion molecule (EpCAM). Remarkably, endothelial cells directly suppress cancer cell mesenchymal properties, as evidenced by a 4.6 fold reduction in Vimentin expression compared to mono-cultures. Additionally, bilateral cancer-endothelial interactions are revealed, aggressive cancer cells induce significant intercellular adhesion molecule-1 (ICAM-1) upregulation in endothelium. The INVADE platform represents an engineering advancement for studying complex cell-cell interactions with implications for understanding metastatic mechanisms.

Indexed as

Cell Line, TumorEpithelial-Mesenchymal TransitionEpithelial Cell Adhesion MoleculeHumansMCF-7 CellsMDA-MB-231 CellsNeoplasm InvasivenessVimentinEpithelial Cell Adhesion MoleculeVimentincancer metastasisendotheliumepithelial‐mesenchymal transitionintravasation, microfluidicsvimentin

Identifiers

PMID40223399
PMCPMC12232236

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.