Evidence map›Paper›PMID 40223397›Full record

ArticlePhysiological reports2025

Myosin relaxation states in skeletal muscle fibers of rats and mice: Effects of sex and adiposity.

Zachery A Roloff, Lien A Phung, Luke A Weyrauch, Philip C Woods, Shawna L McMillin, Brian P Sullivan, Rebecca Barok, Naixin Zhang, Katherine A Murphy, Timothy D O'Connell and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Physiological reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Zachery A RoloffDepartment of Family Medicine and Community Health, Division of Physical Therapy and Rehabilitation Science, University of Minnesota, Minneapolis, Minnesota, USA.
Lien A PhungDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
Luke A WeyrauchDepartment of Family Medicine and Community Health, Division of Physical Therapy and Rehabilitation Science, University of Minnesota, Minneapolis, Minnesota, USA.
Philip C WoodsDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
Shawna L McMillinDepartment of Family Medicine and Community Health, Division of Physical Therapy and Rehabilitation Science, University of Minnesota, Minneapolis, Minnesota, USA.
Brian P SullivanDepartment of Family Medicine and Community Health, Division of Physical Therapy and Rehabilitation Science, University of Minnesota, Minneapolis, Minnesota, USA.
Rebecca BarokDepartment of Family Medicine and Community Health, Division of Physical Therapy and Rehabilitation Science, University of Minnesota, Minneapolis, Minnesota, USA.
Naixin ZhangDepartment of Integrative Biology and Physiology, University of Minnesota, Minneapolis, Minnesota, USA.
Katherine A MurphyDepartment of Integrative Biology and Physiology, University of Minnesota, Minneapolis, Minnesota, USA.
Timothy D O'ConnellDepartment of Integrative Biology and Physiology, University of Minnesota, Minneapolis, Minnesota, USA.
Brendan J DoughertyDepartment of Family Medicine and Community Health, Division of Physical Therapy and Rehabilitation Science, University of Minnesota, Minneapolis, Minnesota, USA.
David D ThomasDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
Mark S MillerDepartment of Kinesiology, University of Massachusetts, Amherst, Massachusetts, USA.
Dawn A LoweDepartment of Family Medicine and Community Health, Division of Physical Therapy and Rehabilitation Science, University of Minnesota, Minneapolis, Minnesota, USA.ORCID 0000-0002-5784-9289

Funding

TRAINING PROGRAM IN MUSCLE RESEARCHT32AR007612 · NIAMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI JAMES M ERVASTI, DAWN A LOWE · 2001 to 2026
$11.3M
Interatction of Estrogen, Age and Activity on Musculoskeletal Strength in FemalesR01AG031743 · NIA · UNIVERSITY OF MINNESOTA · PI DAWN A LOWE · 2009 to 2026
$7.4M
Free fatty acid receptor 4 cardioprotective effects in cardiac ischemic injuryR01HL152215 · NHLBI · UNIVERSITY OF MINNESOTA · PI O'CONNELL, TIMOTHY D, SHEARER, GREGORY C · 2020 to 2024
$3.4M
Estrogen Receptor Signaling in the Expression of Respiratory Motor PlasticityR01HL146477 · NHLBI · UNIVERSITY OF MINNESOTA · PI Brendan J Dougherty · 2021 to 2026
$3.0M
Skeletal muscle protein structural dynamics and function drive applications to drug discoveryR01AR082533 · NIAMS · UNIVERSITY OF MINNESOTA · PI DAWN A LOWE, Robyn T Rebbeck · 2023 to 2026
$2.5M
HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01HL146477HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01HL152215HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) R01AR082533HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) T32AR007612HHS | NIH | National Institute on Aging (NIA) R01AG031743NHLBI NIH HHS R01 HL146477NHLBI NIH HHS R01 HL152215NIAMS NIH HHS R01 AR082533NIAMS NIH HHS T32 AR007612NIA NIH HHS R01 AG031743
6 · The paper itself

Abstract

Myosin disordered- and super-relaxed states (DRX and SRX, respectively) in skeletal muscle fibers are hypothesized to play key roles in thermogenesis and basal metabolic energy expenditure, raising potential for novel therapeutic targets for obesity and other metabolic diseases. Limited studies have investigated relationships between body composition or biological sex and myosin relaxed states. Using fluorescence-based single-nucleotide turnover, we report quantitative relationships of diet-induced adiposity and sex with biochemical parameters of myosin relaxed states of rodent muscle fibers. Our main findings were: (1) adiposity had minimal to no effect on parameters of relaxed myosin states measured in fibers from rats and mice, (2) fibers from female rats and mice had 10%-20% shorter SRX lifetimes than those from males (p ≤ 0.035), (3) in rats, females had shorter DRX lifetimes than males, and (4) myosin heavy chain isoform had negligible impact on parameters of relaxed myosin states. We conclude that skeletal muscle energy utilization during rest, as measured by myosin ATPase, is affected minimally by adiposity, but differs by sex. Continued exploration of the metabolic implications of myosin transitioning between SRX and DRX will provide further understanding of muscle thermogenesis and whole-body metabolism; in so doing, sex as a biological factor should be considered.

Indexed as

AdiposityMuscle Fibers, SkeletalMuscle RelaxationMyosinsAnimalsEnergy MetabolismFemaleMaleMiceMice, Inbred C57BLMyosin Heavy ChainsRatsRats, Sprague-DawleySex CharacteristicsSex FactorsMyosin Heavy ChainsMyosinsadiposityATPasemyosinmyosin heavy chainSRX

Identifiers

PMID40223397
PMCPMC11994889

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.