Evidence map›Paper›PMID 40222732›Full record

ArticleJournal of peptide science : an official publication of the European Peptide Society2025

Penta-ALFA-Tagged Substrates for Self-Labelling Tags Allow Signal Enhancement in Microscopy.

Souvik Ghosh, Ramona Birke, Ashwin Karthick Natarajan, Johannes Broichhagen

Abstract read
In one paragraph

Article in Journal of peptide science : an official publication of the European Peptide Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Souvik GhoshLeibniz-Forschungsinstitut Für Molekulare Pharmakologie (FMP), Berlin, Germany.ORCID https://orcid.org/0000-0003-1224-5008
Ramona BirkeLeibniz-Forschungsinstitut Für Molekulare Pharmakologie (FMP), Berlin, Germany.
Ashwin Karthick NatarajanMax-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin, Germany.ORCID https://orcid.org/0000-0001-7897-708X
Johannes BroichhagenLeibniz-Forschungsinstitut Für Molekulare Pharmakologie (FMP), Berlin, Germany.ORCID https://orcid.org/0000-0003-3084-6595

Funding

European Union's Horizon Europe Framework Programme 101042046
6 · The paper itself

Abstract

Self-labelling proteins like SNAP- and HaloTag have advanced imaging in life sciences by enabling live-cell labeling with fluorophore-conjugated substrates. However, the typical one-fluorophore-per-protein system limits signal intensity. To address this, we developed a strategy using the ALFA-tag system, a 13-amino acid peptide recognized by a bio-orthogonal and fluorescently labelled nanobody, for signal amplification. We synthesized a pentavalent ALFA

Indexed as

Fluorescent DyesPeptidesCarbocyaninesClick ChemistryHEK293 CellsHumansMicroscopy, ConfocalSingle-Domain AntibodiesCarbocyaninescyanine dye 5Fluorescent DyesPeptidesSingle-Domain AntibodiesALFA‐tagcell imagingconfocal microscopyfluorescent labelingnanobodyself‐labelling proteins (SLPs)signal amplification

Identifiers

PMID40222732
PMCPMC11994250

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.