ArticleActa parasitologica2025
Molecular Detection of Benzimidazole Resistance Associated with the F200Y Polymorphism in the β-Tubulin Gene of Ancylostoma caninum: First Report from India.
Article in Acta parasitologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Dopamine signaling drives skin invasion by human-infective nematodes.Nature communications · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe study aimed to detect benzimidazole (BZ) resistance in Ancylostoma caninum in dogs from Thrissur district, Kerala, India, using amplification refractory mutation system- polymerase chain reaction (ARMS-PCR). The primary objective was to determine the presence and frequency of mutations at codon 200 of the isotype-1 β-tubulin gene, associated with BZ resistance, in adult worms and infective larvae.
methodsA total of 75 adult A. caninum worms were collected during post-mortem, and 280 infective larvae (L3) from 35 faecal samples positive for strongyle ova were obtained from various locations. Amplification of the isotype-1 β-tubulin gene at codon 200 was performed by ARMS PCR, followed by Sanger sequencing to confirm of single nucleotide polymorphisms (SNPs). Genotypes were analysed using EMBOSS and NCBI BLAST tools.
resultsAll 75 adult worms exhibited the homozygous susceptible genotype at codon 200. Out of 280 larvae genotyped, two were homozygous resistant, one was heterozygous, and 277 were homozygous susceptible. The overall frequencies of the resistant (TAC) and susceptible (TTC) alleles were 0.01 and 0.99, respectively. Both homozygous and heterozygous resistant larvae were found in a household with recurring ancylostomosis. No mutations were observed at codon 198.
conclusionThe study identified a low frequency of BZ-resistant alleles in A. caninum larvae in Thrissur, Kerala, marking the first such report in India. Although resistance remains at an early stage, ongoing monitoring is essential to mitigate its spread in the canine population.
Indexed as
Identifiers
40221945What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.