Evidence map›Paper›PMID 40221891›Full record

ArticleActa crystallographica. Section D, Structural biology2025

Structure of the Fab fragment of a humanized 5E5 antibody to a cancer-specific Tn-MUC1 epitope.

Wei Li, Ulla Mandel, Henk van Faassen, Matthew J Parker, Max S G Legg, Greg Hussack, Henrik Clausen, Stephen V Evans

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section D, Structural biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Wei LiDepartment of Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, BC V8P 3P6, Canada.ORCID 0000-0003-1249-8945
Ulla MandelCopenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark.
Henk van FaassenHuman Health Therapeutics Research Centre, National Research Council Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada.
Matthew J ParkerDepartment of Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, BC V8P 3P6, Canada.
Max S G LeggDepartment of Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, BC V8P 3P6, Canada.ORCID 0000-0002-9065-7019
Greg HussackHuman Health Therapeutics Research Centre, National Research Council Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada.
Henrik ClausenCopenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark.
Stephen V EvansDepartment of Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, BC V8P 3P6, Canada.ORCID 0000-0002-0366-4027

Funding

Natural Sciences and Engineering Research Council of Canada RGPIN-2020-06021
6 · The paper itself

Abstract

The structure of the humanized Fab from murine monoclonal antibody 5E5 specific for tumor antigen Tn-MUC1 has been determined to 1.57 Å resolution. Despite undertaking thousands of crystallization trials of the humanized 5E5 (h-5E5) Fab in the presence of either the singly or doubly glycosylated peptide antigens corresponding to Tn-MUC1, the Fab is only observed unliganded in the crystal. The conformations of the complementarity-determining regions (CDRs) of the combining site on the h-5E5 Fab do not differ significantly from those reported for liganded murine scFv at 3.0 Å resolution. While the affinity of the murine 5E5 has previously been reported as K

Indexed as

Antibodies, Monoclonal, HumanizedAntigens, Tumor-Associated, CarbohydrateEpitopesImmunoglobulin Fab FragmentsMucin-1AnimalsCrystallography, X-RayGlycosylationHumansMiceModels, MolecularAntibodies, Monoclonal, HumanizedAntigens, Tumor-Associated, CarbohydrateEpitopesImmunoglobulin Fab FragmentsMUC1 protein, humanMucin-1Tn antigen5E5anti-Tn-MUC1 antibodycancerFab structurehumanized antibodiesTn antigenX-ray crystallography

Identifiers

PMID40221891
PMCPMC12054364

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.