Evidence map›Paper›PMID 40221670›Full record

ArticleBMC pregnancy and childbirth2025

An improved procedure based on fluorescence immunochromatography for rapid detection of group B streptococcus from enrichment cultures.

Xiaoli Chen, Sijia Cao, Yan Ni, Xinyi Chen, Yu Qiu, Mingjing Zhang, Jianguo Fu, Lijuan Zheng, Zimin Tang, Huiming Ye

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC pregnancy and childbirth, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiaoli Chen *Department of Laboratory Medicine, Fujian Key Clinical Specialty of Laboratory Medicine, Department of Gynecology and Obstetrics, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China.
Sijia Cao *Department of Laboratory Medicine, Fujian Key Clinical Specialty of Laboratory Medicine, Department of Gynecology and Obstetrics, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China.
Yan Ni *Department of Obstetrics, Department of Gynecology and Obstetrics, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China.
Xinyi ChenDepartment of Laboratory Medicine, Fujian Key Clinical Specialty of Laboratory Medicine, Department of Gynecology and Obstetrics, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China.
Yu QiuDepartment of Obstetrics, Department of Gynecology and Obstetrics, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China.
Mingjing ZhangDepartment of Obstetrics, Department of Gynecology and Obstetrics, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China.
Jianguo FuDepartment of Infection Control, Zhongshan Hospital, Xiamen University, Xiamen, China.
Lijuan ZhengXiamen Innobiomax Biotechnology Company Limited, Xiamen, China.
Zimin TangState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, China. zimintang@xmu.edu.cn.
Huiming YeDepartment of Laboratory Medicine, Fujian Key Clinical Specialty of Laboratory Medicine, Department of Gynecology and Obstetrics, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China. yehuiming@xmu.edu.cn.

Funding

the Major Science and Technology Project of Fujian Provincial Health Commission 2021ZD01006the Medical and Health Guidance Project of Xiamen 3502Z20209207the Natural Science Foundation of Xiamen 3502Z20227138
6 · The paper itself

Abstract

backgroundGroup B streptococcus (GBS) is a major cause of perinatal infectious morbidity and mortality. Although intrapartum antibiotic prophylaxis (IAP) administration for ≥ 4 h is effective in preventing neonatal early-onset GBS diseases, the conventional culture-based approach to identify GBS often takes 24-72 h. This study aimed to find a strategy to improve the efficacy of GBS screening.

methodsWe developed a fluorescence immunochromatographic test (FICT) strip to detect GBS within 15 min. The detection limit, analytical sensitivity, cross-reactivity and performance of the strip were evaluated. The performance of the strip on vaginal-rectal swabs with or without enrichment culture was compared with real-time quantitative polymerase chain reaction (qPCR) and colloidal gold immunochromatography (GIC) assay with conventional enrichment culture method as the reference method.

resultsThe detection limit of the strip ranges from 10

conclusionsThe improved procedure based on the FICT for GBS detection from short-term LIM broth cultures was expected to guide IAP administration in obstetrical emergencies.

Indexed as

Chromatography, AffinityPregnancy Complications, InfectiousStreptococcal InfectionsStreptococcus agalactiaeFemaleFluorescenceHumansLimit of DetectionPregnancyReal-Time Polymerase Chain ReactionRectumSensitivity and SpecificityVaginaFluorescence immunochromatographyGroup B streptococcusMethodological improvementPerformance evaluationRapid test

Identifiers

PMID40221670
PMCPMC11994007

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.