Evidence map›Paper›PMID 40221642›Full record

ArticleBMC microbiology2025

Genome characterization, pathogenicity, and evaluation of therapeutics of Klebsiella aerogenes in Bombyx larvae infection model.

Tansha Kabir, Md Ismail Hossain, Tangerul A Jepu, Mrinmoy Sarker, Nusrat U A Saleh, Hafsa Monir, Nafisa Nawaar, Sadia A Jene, Md Mahtab Hossain, M Aftab Uddin and 2 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Tansha KabirDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
Md Ismail HossainDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
Tangerul A JepuDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
Mrinmoy SarkerDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
Nusrat U A SalehDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
Hafsa MonirDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
Nafisa NawaarDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
Sadia A JeneDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
Md Mahtab HossainDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
M Aftab UddinBangladesh Sericulture Research and Training Institute, Rajshahi, Bangladesh.
M Latiful BariCentre for Advanced Research in Sciences, University of Dhaka, Dhaka, Bangladesh.
Muktadir S HossainDepartment of Biochemistry and Microbiology, School of Health and Life Sciences, North South University, Dhaka, Bangladesh. muktadir.hossain01@northsouth.edu.

Funding

North South University NSU-RP-18-044
6 · The paper itself

Abstract

Antibiotic resistance against human pathogenic bacteria is a global problem and the issue is becoming increasingly serious. Klebsiella aerogenes, a Gram-negative pathogen, is usually found in soil and water, but there are increasing number of reports in on isolation of antibiotic-resistant strains of it. Here, we report the draft genome of a food-borne Klebsiella aerogenes strain isolated from street food of Dhaka, Bangladesh. The WGS analysis revealed the presence of a number of virulence genes and antibiotic-resistance genes. Using the infection model of the larvae of the silk moth, Bombyx mori, we show that the K. aerogenes strain killed larvae within 72 h of injection into the hemolymph (blood) or midgut. Although the strain showed resistance to ampicillin in vitro among the antibiotics tested, it showed sensitivity to ampicillin in vivo in Bombyx larvae. Direct injection of aqueous extracts of hog plum or Indian gooseberry into the midgut of larvae infected with K. aerogenes increased larval survival rate to ~ 75% after 72 h. These results indicate that Bombyx larvae could be used to carry out in vivo screening of plant extracts with potential therapeutic effects against pathogenic bacteria like K. aerogenes.

Indexed as

BombyxGenome, BacterialKlebsiellaKlebsiella InfectionsAnimalsAnti-Bacterial AgentsBangladeshDisease Models, AnimalDrug Resistance, BacterialLarvaMicrobial Sensitivity TestsPlant ExtractsVirulenceVirulence FactorsWhole Genome SequencingAnti-Bacterial AgentsPlant ExtractsVirulence FactorsAntibiotic resistanceBombyx infection modelPathogensPlant extractTherapeutic effect

Identifiers

PMID40221642
PMCPMC11992878

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.