ArticleThe Journal of biological chemistry2025
Mechanism of glutathionylation of the active site thiols of peroxiredoxin 2.
Article in The Journal of biological chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peroxiredoxin 2 (Prdx2) undergoes ready glutathionylation, and glutaredoxin-catalyzed deglutathionylation provides an alternative mechanism to thioredoxin/thioredoxin reductase for recycling the reduced protein (Peskin et al. JBC 216, 3053, 2016). To elucidate the mechanism of glutathionylation, we have carried out kinetic studies using stopped flow and SDS PAGE plus product analysis by mass spectrometry. Kinetic modeling shows a mechanism in which exchange of Prdx2 disulfide with physiological concentrations of GSH occurs over seconds to minutes, initially at one active site to produce glutathionylated dimers linked by one disulfide. Exchange with GSH yields glutathionylation at both the peroxidatic (C
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