Evidence map›Paper›PMID 40220222›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Imaging Protocol for Super-Resolution Microscopy in Fixed T Cells.

Miriam Kopdag, Lola C Hernandez, Anette Rosche, Björn-Philipp Diercks

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Ryanodine Receptor 1 Is Dispensable for CD4European journal of immunology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Miriam KopdagThe Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Lola C HernandezThe Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Anette RoscheThe Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Björn-Philipp DiercksThe Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. b.diercks@uke.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Super-resolution microscopy has revolutionized the field of cellular imaging by overcoming the diffraction limit of conventional light microscopy and enabling the visualization of cellular structures in the nanometre range. In this protocol, a detailed procedure for super-resolution imaging of fixed primary murine T cells is described, focusing on preparation, staining, and post-processing. Imaging is performed using a super-resolution stimulated emission depletion (STED) microscope with a confocal laser scanning unit. After imaging, the data is processed using deconvolution and segmentation to reconstruct high-resolution images that enable detailed analysis of the T cell structures. This method enables a deeper understanding of T cell biology by revealing the intricate details of their internal organization and interactions.

Indexed as

Image Processing, Computer-AssistedT-LymphocytesAnimalsMiceMicroscopy, ConfocalMicroscopy, FluorescenceTissue FixationCalciumImmunofluorescenceIP3ROrai1STEDT cells

Identifiers

PMID40220222

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.