Evidence map›Paper›PMID 40220173›Full record

ArticleBiotechnology letters2025

Engineering the Escherichia coli Nissle strain for monitoring the bacterial cell distribution and therapeutic protein expression within the intestinal tract of animal models.

Rui Gong, Yang Wu, Rushi Liu, Minjing Liao, Meifang Quan

Abstract read
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In one paragraph

Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Rui GongSchool of Medical Technology and Translational Medicine, Health Science Center, Hunan Normal University, Changsha, 410013, China.
Yang WuSchool of Medical Technology and Translational Medicine, Health Science Center, Hunan Normal University, Changsha, 410013, China.
Rushi LiuSchool of Medical Technology and Translational Medicine, Health Science Center, Hunan Normal University, Changsha, 410013, China.
Minjing LiaoSchool of Medical Technology and Translational Medicine, Health Science Center, Hunan Normal University, Changsha, 410013, China.
Meifang QuanKey Laboratory of Molecular Epidemiology of Hunan Province, Health Science Center, Hunan Normal University, Tongzipo Road 371, Changsha, 410013, China. quanmf@hunnu.edu.cn.ORCID http://orcid.org/0000-0003-1784-9687

Funding

Natural Science Foundation of Hunan Province 2023JJ30398Natural Science Foundation of Hunan Province 2023JJ30428
6 · The paper itself

Abstract

The probiotic Escherichia coli Nissle 1917 (EcN) has been developed as a therapeutic carrier capable of enabling in vivo production of functional proteins. To optimize these processes, precise selection of promoters and monitoring of heterologous protein expression are important. Here, we designed a hypoxia-induced expression system in EcN by integrating a bicistronic cassette under the control of the P

Indexed as

Escherichia coliIntestinesAnimalsCarboxy-LyasesGenes, ReporterLuminescent ProteinsMiceProbioticsPromoter Regions, GeneticRecombinant ProteinsRed Fluorescent ProteinCarboxy-LyasesLuminescent ProteinsRecombinant ProteinsRed Fluorescent ProteinCo-expressionEscherichia coli Nissle 1917Hypoxia-inducible promoterMCherryTherapeutic protein

Identifiers

PMID40220173

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.