Gene Knockout in the Developing Brain of Wild-Type Rodents by CRISPR In Utero Electroporation.

Andrea J Romanowski, Ryan R Richardson, Celine Plachez, Reha S Erzurumlu, Alexandros Poulopoulos

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Developmental transformations of Purkinje cells tracked by DNA electrokinetic mobility.Cell reports methods · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Andrea J RomanowskiDepartment of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA.

Ryan R RichardsonDepartment of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA.

Celine PlachezDepartment of Neurobiology, University of Maryland School of Medicine, Baltimore, MD, USA.

Reha S ErzurumluDepartment of Neurobiology, University of Maryland School of Medicine, Baltimore, MD, USA.

Alexandros PoulopoulosDepartment of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA. apoulopoulos@som.umaryland.edu.

Funding

A Lightsheet Microscope for an Established Core FacilityS10OD030221 · OD · UNIVERSITY OF MARYLAND BALTIMORE · PI BLANPIED, THOMAS A · 2021 to 2021

$600k

NIH HHS S10 OD030221

6 · The paper itself

Abstract

CRISPR/Cas9 constructs can be delivered by in utero electroporation to knock out a gene of interest in neurons of the developing brain in wild-type rodents. This approach allows for high-throughput genetic screening, circuit-specific gene knockout, and knockout cell phenotyping using sparse labeling within a wild-type in vivo context. Here we outline the methods and steps of designing guide RNAs in silico, cloning guide RNAs into plasmid backbones, and introducing these plasmids into the developing mouse cortex and hippocampus.

Indexed as

BrainCRISPR-Cas SystemsElectroporationGene Knockout TechniquesAnimalsFemaleHippocampusMiceNeuronsPlasmidsPregnancyRNA, Guide, CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsBrain developmentCRISPRGenome editingIn utero electroporationKnockout

Identifiers

PMID40220102

DOI10.1038/nmeth.3543

What OpenQuestion holds

Textmetadata

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.