ArticleMolecular and cellular biology1985
Mouse testes contain two size classes of actin mRNA that are differentially expressed during spermatogenesis.
Article in Molecular and cellular biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 79 citations in OpenAlex.
- Identification and validation of mouse sperm proteins correlated with epididymal maturation.Proteomics · 2011Article
- Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts.Biology of reproduction · 2008Article
- Developmental and cell type specificity of LINE-1 expression in mouse testis: implications for transposition.Molecular and cellular biology · 1994Article
- Murine beta 1,4-galactosyltransferase: both the amounts and structure of the mRNA are regulated during spermatogenesis.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Stage-specific localization of cytoskeletal actin mRNA in murine seminiferous tubules and intestinal epithelia as demonstrated by in-situ hybridization.Cell and tissue research · 1989Article
- Identification and developmental expression of a smooth-muscle gamma-actin in postmeiotic male germ cells of mice.Molecular and cellular biology · 1989Article
- Expression of a testis-specific hsp70 gene-related RNA in defined stages of rat seminiferous epithelium.The Journal of cell biology · 1988Article
- Isolation of cDNA clones for mouse cytoskeletal gamma-actin and differential expression of cytoskeletal actin mRNAs in mouse cells.Molecular and cellular biology · 1988Article
- Cell-cycle-specific and serum-dependent expression of gamma-actin mRNA in Swiss mouse 3T3 cells.Molecular and cellular biology · 1988Article
- Expression of cellular protooncogenes in the mouse male germ line: a distinctive 2.4-kilobase pim-1 transcript is expressed in haploid postmeiotic cells.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Developmentally regulated expression of a human "finger"-containing gene encoded by the 5' half of the ret transforming gene.Molecular and cellular biology · 1988Article
- Detecting the effects of toxic agents on spermatogenesis using DNA probes.Environmental health perspectives · 1987Review
- Differential expression of the mouse homeobox-containing gene Hox-1.4 during male germ cell differentiation and embryonic development.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- Differential expression of opioid peptide genes by testicular germ cells and somatic cells.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- Expression of c-mos RNA in germ cells of male and female mice.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- c-mos proto-oncogene RNA transcripts in mouse tissues: structural features, developmental regulation, and localization in specific cell types.Molecular and cellular biology · 1987Article
- Developmental-stage-specific expression of the hsp70 gene family during differentiation of the mammalian male germ line.Molecular and cellular biology · 1987Article
- Expression of the proopiomelanocortin gene is developmentally regulated and affected by germ cells in the male mouse reproductive system.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
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3 authors at 1 institution in 1 country.
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Abstract
Using several actin isotype-specific cDNA probes, we found actin mRNA of two size classes, 2.1 and 1.5 kilobases (kb), in extracts of polyadenylated and nonpolyadenylated RNA from sexually mature CD-1 mouse testes. Although the 2.1-kb sequence was present in both meiotic and postmeiotic testicular cell types, it decreased manyfold in late haploid cells. The 1.5-kb actin sequence was not detectable in meiotic pachytene spermatocytes (or in liver or kidney cells), but was present in round and elongating spermatids and residual bodies. To differentiate between the beta- and gamma-actin mRNAs, we isolated a cDNA, pMGA, containing the 3' untranslated region of a mouse cytoplasmic actin that has homology to the 3' untranslated region of a human gamma-actin cDNA but not to the 3' untranslated regions of human alpha-, beta-, or cardiac actins. Dot blot hybridizations with pMGA detected high levels of presumptive gamma-actin mRNA in pachytene spermatocytes and round spermatids, with lower amounts found in elongating spermatids. Hybridization with the 3' untranslated region of a rat beta-actin probe revealed that round spermatids contained higher levels of beta-actin mRNA than did pachytene spermatocytes or residual bodies. Both probes hybridized to the 2.1-kb actin mRNA but failed to hybridize to the 1.5-kb mRNA.
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