Evidence map›Paper›PMID 40220094›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Prime Editing of Mouse Primary Neurons.

Colin D Robertson, Ryan R Richardson, Marilyn Steyert, Corinne A Martin, Corey Flynn, Alexandros Poulopoulos

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Gene-sized editing for the therapy of genetic diseases.Functional & integrative genomics · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Colin D RobertsonDepartment of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA.
Ryan R RichardsonDepartment of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA.
Marilyn SteyertDepartment of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA.
Corinne A MartinDepartment of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA.
Corey FlynnAtomic Insights, Inc, Poway, CA, USA.
Alexandros PoulopoulosDepartment of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA. apoulopoulos@som.umaryland.edu.

Funding

Untangling the biology of brain wiring: high-throughput innovations for molecular screening and functional targeting of circuit development in vivoDP2MH122398 · NIMH · UNIVERSITY OF MARYLAND BALTIMORE · PI POULOPOULOS, ALEXANDROS · 2019 to 2019
$2.3M
NIMH NIH HHS DP2 MH122398
6 · The paper itself

Abstract

Prime editing is a hybrid genome editing technology that introduces small edits on the genome with high precision. It combines nickase Cas9 with reverse transcriptase to prime and synthesizes edited DNA from RNA, reducing unintended insertions and deletions on the genome. This protocol describes the design of prime editing guide RNAs (pegRNAs), cloning of plasmids, nucleofection of mouse primary neurons, and preparation for next-generation sequencing. Directions are given for pegRNA and PE3b gRNA design and construction using PegAssist, a publicly available webtool and plasmid set. Prime editing in neurons allows genome manipulation while maintaining endogenous gene expression, making it ideal for studying protein structure/function relationships and pathogenic variants in a native neuronal context.

Indexed as

Gene EditingNeuronsAnimalsCells, CulturedCRISPR-Cas SystemsHigh-Throughput Nucleotide SequencingMicePlasmidsRNA, Guide, CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsCRISPRGenome editingNeuronsNucleofectionPegAssistpegRNAPrime editing

Identifiers

PMID40220094

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.