Evidence map›Paper›PMID 40220072›Full record

ArticleAnalytical and bioanalytical chemistry2025

A colorimetric sandwich assay based on magnetic separation of Brevinin-1BW-functionalized magnetic beads and porcine IgG for the detection of Staphylococcus aureus.

Dongxia He, Qi Liu, Lei Wang, Qinqin Han, Jinyang Zhang, Chao Li, Yuzhu Song

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dongxia HeFaculty of Life Science and Technology, Kunming University of Science and Technology, No.727, Jingming South Road, Chenggong District, Kunming City, 650500, China.
Qi LiuFaculty of Life Science and Technology, Kunming University of Science and Technology, No.727, Jingming South Road, Chenggong District, Kunming City, 650500, China.
Lei WangFaculty of Life Science and Technology, Kunming University of Science and Technology, No.727, Jingming South Road, Chenggong District, Kunming City, 650500, China.
Qinqin HanFaculty of Life Science and Technology, Kunming University of Science and Technology, No.727, Jingming South Road, Chenggong District, Kunming City, 650500, China.
Jinyang ZhangFaculty of Life Science and Technology, Kunming University of Science and Technology, No.727, Jingming South Road, Chenggong District, Kunming City, 650500, China.
Chao LiFaculty of Life Science and Technology, Kunming University of Science and Technology, No.727, Jingming South Road, Chenggong District, Kunming City, 650500, China.
Yuzhu SongFaculty of Life Science and Technology, Kunming University of Science and Technology, No.727, Jingming South Road, Chenggong District, Kunming City, 650500, China. yuzhusong@kust.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel strategy integrating Brevinin-1BW (BW)-functionalized magnetic bead-based separation with a colorimetric immunoassay was developed to concentrate and enrich Staphylococcus aureus (S. aureus) from sample matrices using magnetic beads functionalized with antimicrobial peptide BW (MBs-BW) as carriers. To ensure the selectivity of the method, horseradish peroxidase (HRP)-labeled porcine IgG (HRP-porcine IgG) was used as a molecular recognition reagent and signal amplification probe. MBs-BW/S. aureus/HRP-porcine IgG probe sandwich complex was successfully obtained. HRP chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) was used to realize the chromogenic reaction. Finally, S. aureus was quantitatively analyzed based on the color signal generated. Under the optimal conditions, the assay showed a response in the concentration range of 1.0 × 10

Indexed as

Antimicrobial Cationic PeptidesColorimetryImmunoglobulin GImmunomagnetic SeparationStaphylococcus aureusAnimalsImmunoassayLimit of DetectionSwineAntimicrobial Cationic PeptidesImmunoglobulin GBrevinin-1BWColorimetric immunoassayMagnetic beadsMammalian IgGStaphylococcus aureus

Identifiers

PMID40220072

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.