Evidence map›Paper›PMID 40219064›Full record

ArticlePlants (Basel, Switzerland)2025

A Protoplast System for CRISPR-Cas Ribonucleoprotein Delivery in

Barbara M Marques, Daniel B Sulis, Bethany Suarez, Chenmin Yang, Carlos Cofre-Vega, Robert D Thomas, Justin G A Whitehill, Ross W Whetten, Rodolphe Barrangou, Jack P Wang

Abstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Barbara M MarquesForest Biotechnology Group, North Carolina State University, Raleigh, NC 27695, USA.ORCID 0000-0002-1427-6556
Daniel B SulisForest Biotechnology Group, North Carolina State University, Raleigh, NC 27695, USA.ORCID 0000-0002-6657-9567
Bethany SuarezForest Biotechnology Group, North Carolina State University, Raleigh, NC 27695, USA.
Chenmin YangForest Biotechnology Group, North Carolina State University, Raleigh, NC 27695, USA.
Carlos Cofre-VegaForest Biotechnology Group, North Carolina State University, Raleigh, NC 27695, USA.ORCID 0000-0002-5272-5009
Robert D ThomasBiotechnology Teaching Group, North Carolina State University, Raleigh, NC 27695, USA.
Justin G A WhitehillDepartment of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695, USA.
Ross W WhettenDepartment of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695, USA.
Rodolphe BarrangouTreeCo, Raleigh, NC 27606, USA.ORCID 0000-0002-0648-3504
Jack P WangForest Biotechnology Group, North Carolina State University, Raleigh, NC 27695, USA.

Funding

Cooperative State Research Service of the U.S. Department of Agriculture NCZ04214North Carolina Specialty Crop Block Grants 20-070-4013North Carolina State University Chancellor's Innovation Fund 190549MAU.S. National Science Foundation Small Business Technology Transfer Program 2044721
6 · The paper itself

Abstract

Climate change profoundly impacts the health, productivity, and resilience of forest ecosystems and threatens the sustainability of forest products and wood-based industries. Innovations to enhance tree growth, development, and adaptation offer unprecedented opportunities to strengthen ecosystem resilience and mitigate the effects of climate change. Here, we established a method for protoplast isolation, purification, and CRISPR-Cas ribonucleoprotein (RNP) delivery in

Indexed as

conifersCRISPRgenome editingprotoplasts

Identifiers

PMID40219064
PMCPMC11990275

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.