Multiplex imaging of murine bone marrow using Phenocycler 2.0™.
Sonali J Karnik, Connor Gulbronson, Paige C Jordan, Rahul Kanumuri, Baskar Ramdas, Ramesh Kumar, Melissa L Hartman, Izza Khurram, Drew M Brown, Karen E Pollok and 3 more
Article in Leukemia, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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5 · Who and what money
Authors and funding
13 authors.
Sonali J Karnik *Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.ORCID 0000-0002-1905-2885
Connor Gulbronson *Indiana University Cooperative Center of Excellence in Hematology (CCEH), Indianapolis, IN, USA.
Paige C JordanDepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
Rahul KanumuriIndiana University Cooperative Center of Excellence in Hematology (CCEH), Indianapolis, IN, USA.
Baskar RamdasIndiana University Cooperative Center of Excellence in Hematology (CCEH), Indianapolis, IN, USA.ORCID 0009-0006-2206-5720
Ramesh KumarIndiana University Cooperative Center of Excellence in Hematology (CCEH), Indianapolis, IN, USA.ORCID 0000-0002-7545-4619
Melissa L HartmanDepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
Izza KhurramDepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
Drew M BrownIndiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, IN, USA.
Karen E PollokIndiana University Cooperative Center of Excellence in Hematology (CCEH), Indianapolis, IN, USA.
Pratibha SinghIndiana University Cooperative Center of Excellence in Hematology (CCEH), Indianapolis, IN, USA.
Reuben KapurIndiana University Cooperative Center of Excellence in Hematology (CCEH), Indianapolis, IN, USA. rkapur@iu.edu.ORCID 0000-0003-2225-1700
Melissa A KacenaDepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. mkacena@iu.edu.ORCID 0000-0001-7293-0088
Funding
Tumor Microenvironment and Metastasis ProgramP30CA082709 · NCI · INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS · PI David W Clapp · 1999 to 2026
$59.3M
Indiana Clinical and Translational Sciences InstituteUL1TR001108 · NCATS · INDIANA UNIVERSITY INDIANAPOLIS · PI DENNE, SCOTT C., SHEKHAR, ANANTHA · 2013 to 2017
$23.3M
Project-005U54DK106846 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI Reuben Kapur, Karen Elizabeth Pollok · 2015 to 2026
$9.7M
Hematology Central Coordinating Center (HCCC) for the NIDDK Hematology Centers ProgramU24DK126127 · NIDDK · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI Anna E Beaudin, DEREK L STIREWALT · 2020 to 2026
$7.5M
Role of p21 activated kinase in LeukemogenesisR01CA173852 · NCI · INDIANA UNIVERSITY INDIANAPOLIS · PI KAPUR, REUBEN · 2014 to 2025
$3.4M
Role of Shp2 in FLT3-ITD-Induced LeukemogenesisR01CA134777 · NCI · INDIANA UNIVERSITY INDIANAPOLIS · PI KAPUR, REUBEN · 2011 to 2023
$3.4M
Systems Biology and Multi-Omics Core (SBMO)P30AR083854 · NIAMS · INDIANA UNIVERSITY INDIANAPOLIS · PI ALEXANDER G ROBLING · 2024 to 2026
$2.8M
CD166 Regulates Human and Murine Hematopoietic Stem Cell Function and the Hematopoietic NicheR01DK108342 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI KACENA, MELISSA A · 2019 to 2023
$1.8M
Osteomacs and megakaryocytes interact to regulate hematopoietic stem cell functionR01DK118782 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI KACENA, MELISSA A, SROUR, EDWARD F · 2019 to 2021
$951k
Osteomacs and megakaryocytes interact to regulate hematopoietic stem cell functionR01AG082275 · NIA · INDIANA UNIVERSITY INDIANAPOLIS · PI KACENA, MELISSA A · 2022 to 2023
$634k
CD166 Regulates Human and Murine Hematopoietic Stem Cell Function and the Hematopoietic NicheR56DK108342 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI KACENA, MELISSA A, SROUR, EDWARD F · 2015 to 2015
Bone marrow (BM) is a tissue that is of great importance to several areas of basic and translational research, including hematology, oncology, bone biology, and immunology. It is unique in that it is gelatinous in nature but housed in a hard casing of bone. Traditionally, flow cytometry and immunofluorescence (IF) techniques have been employed to study the composition of cellular interactions and elements of the BM. However, it has been challenging to study the BM in an unperturbed state using multiple fluorescent probes at a time to fully appreciate the diverse cell populations and their interactions and relative positioning with each other. This protocol addresses how Phenocycler 2.0
Indexed as
Bone MarrowImage Processing, Computer-AssistedAnimalsBone Marrow CellsFlow CytometryMiceSoftware
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Multiplex imaging of murine bone marrow using Phenocycler 2.0™. · full record | OpenQuestion