ArticleScientific reports2025
A rapid and high-yield method for nucleic acid extraction.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Analysis of cell-free DNA in lymphomas: from sample collection to genotyping and minimal residual disease monitoring.Blood advances · 2026Review
- Near-real-time whole-genome sequencing in hospital infection control: a narrative review.Infection · 2026Review
- Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR.Poultry science · 2026Article
- Chemical and Technological Aspects of Magnetic Particles for Cancer Liquid Biopsy Applications.ChemistryOpen · 2026Review
- Green and ultrafast nucleic acid extraction via sphere-mediated confinement ultrasonic grinding for sensitive foodborne pathogen detection.Ultrasonics sonochemistry · 2026Article
- Trends of nucleic acid - based point-of-care diagnostics for infectious diseases.Journal of biological engineering · 2026Review
- Research progress on nucleic acid amplification-based detection technologies for phytopathogenic fungi.Applied microbiology and biotechnology · 2026Review
- Biomimetic Fermentation Reshapes Precursor Pools to Drive Synergistic Roasting Reactions and Enhance Coffee Flavor Complexity.Foods (Basel, Switzerland) · 2026Article
- Multiphysics model for designing magnetic bead collection processes on centrifugal microfluidic platforms.RSC advances · 2026Article
- Comparison of boiling versus magnetic bead techniques in nucleic acid extraction for human papillomavirus detection: evidence based 17,179 cases.Virology journal · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a magnetic silica bead-based nucleic acid extraction method which is rapid (6 - 7 min) and efficient (extracts nearly all the nucleic acid in the sample). We call this method SHIFT-SP (Silica bead based HIgh yield Fast Tip based Sample Prep). Factors such as pH during binding of nucleic acid to bead, the mode of bead movement during binding, the duration of binding, pH, temperature and duration of elution affect the final yield of the method. We have compared the method developed here to commercially available bead and column-based methods. The commercially available bead-based method took about 40 minutes from lysed sample to extracted nucleic acid and had a similar DNA yield as SHIFT-SP. The commercially available column-based method took 25 minutes with half the DNA yield as SHIFT-SP. In addition to a favorable time and yield, all steps in this developed method are automation compatible. We demonstrated a similar efficiency and speed of extraction for both DNA and RNA and showed that the performance of SHIFT-SP was unaffected by DNA size. SHIFT-SP was also able to extract DNA from low concentrations of microbes from enriched whole blood, for downstream whole genome amplification and sequencing.
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Registered trials
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