Evidence map›Paper›PMID 40215966›Full record

ArticleNeuron2025

An integrated microfluidic and fluorescence platform for probing in vivo neuropharmacology.

Sean C Piantadosi, Min-Kyu Lee, Mingzheng Wu, Huong Huynh, Raudel Avila, Catalina A Zamorano, Carina Pizzano, Yixin Wu, Rachael Xavier, Maria Stanslaski and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Sean C PiantadosiDepartment of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA; Center of Excellence in Neurobiology of Addiction, Pain, and Emotion (NAPE), University of Washington, Seattle, WA, USA.
Min-Kyu LeeQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA; Neurolux Inc., Northfield, IL 60093, USA; Shirley Ryan AbilityLab, Chicago, IL 60611, USA; Department of Physical Medicine and Rehabilitation, Northwestern University, Chicago, IL 60611, USA.
Mingzheng WuQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA; Department of Neurobiology, Northwestern University, Evanston, IL, USA.
Huong HuynhQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA; Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.
Raudel AvilaDepartment of Mechanical Engineering, Rice University, Houston, TX, USA; Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA.
Catalina A ZamoranoDepartment of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA; Center of Excellence in Neurobiology of Addiction, Pain, and Emotion (NAPE), University of Washington, Seattle, WA, USA; Department of Pharmacology, University of Washington, Seattle, WA, USA.
Carina PizzanoDepartment of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA; Center of Excellence in Neurobiology of Addiction, Pain, and Emotion (NAPE), University of Washington, Seattle, WA, USA.
Yixin WuQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA.
Rachael XavierNeurolux Inc., Northfield, IL 60093, USA.
Maria StanslaskiNeurolux Inc., Northfield, IL 60093, USA.
Jiheon KangQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA.
Sarah ThaiDepartment of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA; Center of Excellence in Neurobiology of Addiction, Pain, and Emotion (NAPE), University of Washington, Seattle, WA, USA.
Youngdo KimQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA.
Jinglan ZhangDepartment of Neurobiology, Northwestern University, Evanston, IL, USA.
Yonggang HuangQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA; Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208, USA.
Yevgenia KozorovitskiyDepartment of Neurobiology, Northwestern University, Evanston, IL, USA; Chemistry of Life Processes Institutes, Northwestern University, Evanston, IL, USA.
Cameron H GoodQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA; Neurolux Inc., Northfield, IL 60093, USA.
Anthony R BanksQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA; Neurolux Inc., Northfield, IL 60093, USA.
John A RogersQuerrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA; Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA; Department of Neurological Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. Electronic address: jrogers@northwestern.edu.
Michael R BruchasDepartment of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA; Center of Excellence in Neurobiology of Addiction, Pain, and Emotion (NAPE), University of Washington, Seattle, WA, USA; Department of Pharmacology, University of Washington, Seattle, WA, USA; Department of Bioengineering, University of Washington, Seattle, WA, USA. Electronic address: mbruchas@uw.edu.

Funding

University of Washington Center of Excellence in Opioid Addiction ResearchP30DA048736 · NIDA · UNIVERSITY OF WASHINGTON · PI Charles Chavkin · 2019 to 2026
$13.0M
Decoding Locus Coeruleus Neural Circuits and Signaling In Negative AffectR01MH112355 · NIMH · WASHINGTON UNIVERSITY · PI Michael R. Bruchas · 2016 to 2026
$5.3M
Anesthesiology & Perioperative Medicine Training GrantT32GM086270 · NIGMS · UNIVERSITY OF WASHINGTON · PI Tonya M Palermo · 2009 to 2026
$4.3M
Dynamic entanglements: the functional role and mechanistic basis of inter-individual neural synchronyU01NS131406 · NINDS · NORTHWESTERN UNIVERSITY · PI DONALDSON, ZOE REBECCA, KOZOROVITSKIY, YEVGENIA · 2023 to 2025
$4.2M
Next-gen Opto-GPCRs: spatiotemporal simulation of neuromodulator signalingR01MH111520 · NIMH · WASHINGTON UNIVERSITY · PI BRUCHAS, MICHAEL R., SUNAHARA, ROGER K · 2016 to 2018
$3.2M
Neurohypophyseal regulation of midbrain dopamine systems.R01MH117111 · NIMH · NORTHWESTERN UNIVERSITY · PI KOZOROVITSKIY, YEVGENIA · 2018 to 2022
$2.0M
Wireless, implantable optofluidic systems for programmed pharmacology and optogeneticsR42MH116525 · NIMH · NEUROLUX, INC. · PI GHAFFARI, ROOZBEH · 2019 to 2020
$1.4M
Optopharmacology and Sensors for Dissecting Opioid Action In VivoR33DA051489 · NIDA · UNIVERSITY OF WASHINGTON · PI BRUCHAS, MICHAEL R., GU, LIANGCAI · 2023 to 2024
$1.0M
The role of valence encoding amygdala ensembles in avoidance behaviorK99DA062328 · NIDA · UNIVERSITY OF WASHINGTON · PI Sean Christopher Piantadosi · 2025 to 2026
$297k
Isolating the role of endogenous mu-opioid activity in the VTA during natural rewardF31DA059186 · NIDA · UNIVERSITY OF WASHINGTON · PI ZAMORANO, CATALINA ALEJANDRA · 2023 to 2025
$146k
NIDA NIH HHS F31 DA059186NIDA NIH HHS K99 DA062328NIDA NIH HHS P30 DA048736NIDA NIH HHS R33 DA051489NIGMS NIH HHS T32 GM086270NIMH NIH HHS R01 MH111520NIMH NIH HHS R01 MH112355NIMH NIH HHS R01 MH117111NIMH NIH HHS R42 MH116525NINDS NIH HHS U01 NS131406
6 · The paper itself

Abstract

Neurotechnologies and genetic tools for dissecting neural circuit functions have advanced rapidly over the past decade although the development of complementary pharmacological methodologies has comparatively lagged. Understanding the precise pharmacological mechanisms of neuroactive compounds is critical for advancing basic neurobiology and neuropharmacology, as well as for developing more effective treatments for neurological and neuropsychiatric disorders. However, integrating modern tools for assessing neural activity in large-scale neural networks with spatially localized drug delivery remains a major challenge. Here, we present a dual microfluidic-photometry platform that enables simultaneous intracranial drug delivery with neural dynamics recording in the rodent brain. The integrated platform combines a wireless, battery-free, miniaturized fluidic microsystem with optical probes, allowing for spatially and temporally restricted drug delivery while sensing activity-dependent fluorescence using genetically encoded calcium indicators (GECIs), neurotransmitter sensors, and neuropeptide sensors. We demonstrate the performance of this platform for investigating neuropharmacological mechanisms in vivo in behaving mice.

Indexed as

BrainMicrofluidic Analytical TechniquesMicrofluidicsNeuropharmacologyAnimalsDrug Delivery SystemsMiceMice, Inbred C57BLbiosensorfiber photometryintracranial drug deliverymicrofluidicneuropharmacologyoptogeneticsrodent behavior

Identifiers

PMID40215966
PMCPMC12097939

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.