Evidence map›Paper›PMID 40214951›Full record

ArticleBiological trace element research2025

Transcriptome Analysis Reveals Anti-inflammatory, Antiapoptotic, and Milk Synthesis-Promoting Effects of Lithium Chloride in Bovine Mammary Epithelial Cells.

Junhao Cui, Dongqiao Peng, Jinxin Zong, Jinge Zhang, Xinyue Yang, Yongcheng Jin

Abstract read
PubMed Publisher
In one paragraph

Article in Biological trace element research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Iranian journal of medical sciences · 2026
    Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Junhao Cui *Jilin Provincial key laboratory of livestock and poultry feed and feeding in northeastern frigid area, Department of Animal Science, College of Animal Science, Jilin University, Changchun, 130062, China.ORCID http://orcid.org/0000-0002-8793-4601
Dongqiao Peng *Jilin Provincial key laboratory of livestock and poultry feed and feeding in northeastern frigid area, Department of Animal Science, College of Animal Science, Jilin University, Changchun, 130062, China.ORCID http://orcid.org/0000-0002-0408-0381
Jinxin ZongJilin Provincial key laboratory of livestock and poultry feed and feeding in northeastern frigid area, Department of Animal Science, College of Animal Science, Jilin University, Changchun, 130062, China.ORCID http://orcid.org/0000-0002-1369-975X
Jinge ZhangJilin Provincial key laboratory of livestock and poultry feed and feeding in northeastern frigid area, Department of Animal Science, College of Animal Science, Jilin University, Changchun, 130062, China.ORCID http://orcid.org/0009-0009-5792-091X
Xinyue YangJilin Provincial key laboratory of livestock and poultry feed and feeding in northeastern frigid area, Department of Animal Science, College of Animal Science, Jilin University, Changchun, 130062, China.ORCID http://orcid.org/0009-0003-5963-6468
Yongcheng JinJilin Provincial key laboratory of livestock and poultry feed and feeding in northeastern frigid area, Department of Animal Science, College of Animal Science, Jilin University, Changchun, 130062, China. ycjin@jlu.edu.cn.ORCID http://orcid.org/0000-0002-3462-3674

Funding

Changchun Science and Technology Key R&D Program Funding Project, China 21ZGN21Science and Technology Development Plan Project of Jilin Province, China 202202002053NC
6 · The paper itself

Abstract

Lithium, an essential trace element in higher animals, has anti-inflammatory, anti-apoptotic, and nutritional roles. The mechanism by which lithium chloride (LiCl) promotes milk synthesis has been partially elucidated in our previous study; however, potential regulators remain to be identified, and the potential properties of LiCl to inhibit inflammation and apoptosis in bovine mammary epithelial (MAC-T) cells are yet to be clarified. This study aimed to investigate the effects of LiCl on MAC-T cells induced by differentiation using RNA sequencing technology. A total of 237 differentially expressed genes (DEGs) were identified. Through functional annotation using gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis, we identified several genes associated with LiCl function. These genes include inflammation-related genes such as FOSB, C1QTNF1, ANKRD1, DUSP10, ATF3, FAP, and FGF18; apoptosis-related genes such as FAP, FGF18, TMEM170B, and TBX20; milk fat synthesis-related gene FABP9; and milk protein synthesis-related genes TMEM170B, TBX20, FCER1G, and C1QTNF1. Additionally, we found KRT35, a gene that may promote cell proliferation. The aforementioned genes were confirmed via qPCR, and the expression levels of inflammation-related genes pointing to NF-κB (p65) and cellular pro-inflammatory factors TNFα, IL- 1β, IL- 6, and IL- 8 were determined. Following a comprehensive analysis, we hypothesized that in MACT cells, LiCl could inhibit the transcription of inflammatory cytokines genes through inhibition of NF-κB and MAPK signaling pathways to exert anti-inflammatory effects, and increase milk protein and milk fat synthesis through Wnt/β-catenin and mTOR signaling pathways through the differences in gene expression.

Indexed as

Anti-Inflammatory AgentsApoptosisEpithelial CellsGene Expression ProfilingLithium ChlorideMammary Glands, AnimalMilkTranscriptomeAnimalsCattleFemaleAnti-Inflammatory AgentsLithium ChlorideBovine mammary epithelial cellsInflammationLithium chlorideMilk fatMilk proteinTranscriptome

Identifiers

PMID40214951

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.