Evidence map›Paper›PMID 40214501›Full record

ArticleCells2025

The Ceramide-Dependent EV Secretome Differentially Affects Prostate Cancer Cell Migration.

Dolma Choezom, Jan-Moritz Plum, Pradhipa Karuna M, Adi Danieli-Mackay, Christof Lenz, Phillipp Brockmeyer, Julia Christina Gross

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dolma ChoezomDepartment of Hematology and Oncology, University Medical Center Goettingen, 37075 Goettingen, Germany.
Jan-Moritz PlumDepartment of Hematology and Oncology, University Medical Center Goettingen, 37075 Goettingen, Germany.
Pradhipa Karuna MDepartment of Hematology and Oncology, University Medical Center Goettingen, 37075 Goettingen, Germany.ORCID 0000-0002-7934-7407
Adi Danieli-MackayDepartment of Hematology and Oncology, University Medical Center Goettingen, 37075 Goettingen, Germany.ORCID 0000-0001-7337-3797
Christof LenzDepartment of Clinical Chemistry, University Medical Center Goettingen, 37075 Goettingen, Germany.ORCID 0000-0002-0946-8166
Phillipp BrockmeyerDepartment of Oral and Maxillofacial Surgery, University Medical Center Goettingen, 37075 Goettingen, Germany.ORCID 0000-0003-3694-2828
Julia Christina GrossInstitute of Molecular Medicine, Department Medicine, HMU Health and Medical University Potsdam, 14471 Potsdam, Germany.ORCID 0000-0002-8939-5664

Funding

Deutsche Forschungsgemeinschaft GR4810/2-1Deutsche Forschungsgemeinschaft SFB1324/1
6 · The paper itself

Abstract

Tumor-derived extracellular vesicles (EVs) play an important role in cancer progression. Neutral sphingomyelinases (nSMases) are lipid-modifying enzymes that modulate the secretion of EVs from cells. How nSMase activity and therefore ceramide generation affect the composition and functionality of secreted EVs is not fully understood. Here, we aimed to investigate the expression of nSMases 1 and 2 in prostate cancer (PCa) tissue and their role in EV composition and secretion for prostate cancer cell migration. Reduced nSMase 1 and 2 expression was found in prostate cancer and correlated with the age of the patient. When nSMase 2 was inhibited by GW4869 in PCa cells (PC3 and DU145), the EV secretome was significantly altered, while the number of EVs and the total protein content of released EVs were not significantly changed. Using proteomic analysis, we found that extracellular matrix proteins, such as SDC4 (Syndecan-4) and SRPX-2, were differentially secreted on EVs from GW4869-treated PC3 cells. In scratch wound migration assays, GW4869 significantly increased migration compared to control PC3 cells but not DU145 cells, while SDC4 knockdown significantly reduced the migration of PC3 cells. These and other nSMase-2-dependent secreted proteins are interesting candidates for understanding the role of stress-induced EVs in the progression of prostate cancer.

Indexed as

Cell MovementCeramidesExtracellular VesiclesProstatic NeoplasmsSecretomeAniline CompoundsBenzylidene CompoundsCell Line, TumorHumansMalePC-3 CellsSphingomyelin PhosphodiesteraseAniline CompoundsBenzylidene CompoundsCeramidesGW 4869Sphingomyelin Phosphodiesterasecell migrationextracellular matrixneutral sphingomyelinasesSMPD2SMPD3

Identifiers

PMID40214501
PMCPMC11988362

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.