Evidence map›Paper›PMID 40214444›Full record

ArticleCells2025

TNF-Alpha Inhibitor Prevents Cigarette Smoke Extract-Induced Cell Death in Osteoarthritis-Derived Chondrocytes in Culture.

Débora Levy, Alexandra Fernandes Calllera, Alyne Riani Moreira, Iolanda de Fátima Lopes Calvo Tibério, Pedro Nogueira Giglio, Marco Kawamura Demange, Sergio Paulo Bydlowski, Fernanda Degobbi Tenorio Quirino Dos Santos Lopes

Abstract read
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Débora LevyLipids, Oxidation, and Cell Biology Group, Laboratory of Immunology (LIM19), Heart Institute (InCor), Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-010, Brazil.ORCID 0000-0001-7492-997X
Alexandra Fernandes CallleraLaboratory of Experimental Therapeutics, LIM-20, Department of Clinical Medicine, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, Brazil.ORCID 0009-0009-9278-2743
Alyne Riani MoreiraLaboratory of Experimental Therapeutics, LIM-20, Department of Clinical Medicine, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, Brazil.
Iolanda de Fátima Lopes Calvo TibérioLaboratory of Experimental Therapeutics, LIM-20, Department of Clinical Medicine, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, Brazil.ORCID 0000-0002-5662-7895
Pedro Nogueira GiglioInstitute of Orthopedics and Traumatology, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-010, Brazil.
Marco Kawamura DemangeInstitute of Orthopedics and Traumatology, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-010, Brazil.
Sergio Paulo BydlowskiLipids, Oxidation, and Cell Biology Group, Laboratory of Immunology (LIM19), Heart Institute (InCor), Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-010, Brazil.ORCID 0000-0002-7582-5942
Fernanda Degobbi Tenorio Quirino Dos Santos LopesLaboratory of Experimental Therapeutics, LIM-20, Department of Clinical Medicine, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, Brazil.ORCID 0000-0002-6402-3055

Funding

CNPq 302957/2021-9CNPq 305990/2020-9
6 · The paper itself

Abstract

Smoking has been associated, among other factors, with musculoskeletal disorders. Although there is no consensus about the effects of smoking on osteoarthritis (OA), the increase in TNF-alpha in smokers has been considered an important factor in OA induction or progression. However, studies on the effects of smoking on chondrocytes are lacking. Here we aimed to study the effects of cigarette smoke extract (CSE) associated with a TNF-alpha inhibitor on cell death of primary human chondrocytes derived from osteoarthritic patients. CSE at 10% led to cell death by apoptosis after 48 h of incubation, together with caspase 3/7 activation, decrease in mitochondrial transmembrane potential, ROS production, and improvement in

Indexed as

ChondrocytesOsteoarthritisSmokeTumor Necrosis Factor-alphaAgedApoptosisCaspase 3Caspase 7Cell DeathCells, CulturedCore Binding Factor Alpha 1 SubunitHumansMaleMembrane Potential, MitochondrialMiddle AgedReactive Oxygen SpeciesCaspase 3Caspase 7Core Binding Factor Alpha 1 SubunitReactive Oxygen SpeciesSmokeSOX9 Transcription FactorTumor Necrosis Factor-alphaapoptosiscaspase 3/7chondrocytecigarette smoke extractmitochondrial transmembrane potentialosteoarthritisROSTNF-α inhibitor

Identifiers

PMID40214444
PMCPMC11988143

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.