ArticleNpj imaging2025
Three-dimensional fiber orientation mapping of ex vivo human brain at micrometer resolution.
Article in Npj imaging, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Scaling up polarization-sensitive optical coherence tomography to image the whole macaque brain.bioRxiv : the preprint server for biology · 2026Article
- Bridging the microstructural gap in human connectomics using hierarchical phase-contrast tomography as a reference for diffusion MRI in the human brain.bioRxiv : the preprint server for biology · 2026Article
- Measurement of the absolute value of the optical birefringence of myelin in primate brain tissue.Neurophotonics · 2026Article
- The modular nature of long-association pathways in the human brain.bioRxiv : the preprint server for biology · 2026Article
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14 authors.
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Abstract
The accurate measurement of three-dimensional (3D) fiber orientation in the brain is crucial for reconstructing fiber pathways and studying their involvement in neurological diseases. Comprehensive reconstruction of axonal tracts and small fascicles requires high-resolution technology beyond the ability of current in vivo imaging (e.g., diffusion magnetic resonance imaging). Optical imaging methods such as polarization-sensitive optical coherence tomography (PS-OCT) can quantify fiber orientation at micrometer resolution but have been limited to two-dimensional in-plane orientation, preventing the comprehensive study of connectivity in 3D. In this work we present a novel method to quantify volumetric 3D orientation in full angular space with PS-OCT in postmortem human brain tissues. We measure the polarization contrasts of the brain sample from two illumination angles of 0 and 15° and apply a computational method that yields the 3D optic axis orientation and true birefringence. We further present 3D fiber orientation maps of entire coronal cerebrum sections and brainstem with 10 μm in-plane resolution, revealing unprecedented details of fiber configurations. We envision that our method will open a promising avenue towards large-scale 3D fiber axis mapping in the human brain as well as other complex fibrous tissues at microscopic level.
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