Short-loop engineering strategy for enhancing enzyme thermal stability.

Wenlong Zhu, Yiheng Liu, Hui Cao, Luo Liu, Tianwei Tan

Abstract read

In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

SegDesign: A modular framework for controllable protein segment engineering.Protein science : a publication of the Protein Society · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Wenlong ZhuNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, No. 15 North 3rd Ring East Road, Beijing 100029, P.R. China.

Yiheng LiuNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, No. 15 North 3rd Ring East Road, Beijing 100029, P.R. China.

Hui CaoNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, No. 15 North 3rd Ring East Road, Beijing 100029, P.R. China.

Luo LiuNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, No. 15 North 3rd Ring East Road, Beijing 100029, P.R. China.

Tianwei TanNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, No. 15 North 3rd Ring East Road, Beijing 100029, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Highly flexible regions were targeted for successful modification to enhance enzyme stability. However, this approach could not cover all key sites. Residues in certain rigid regions are also crucial for protein stability. This study proposed a short-loop engineering strategy that explores rigid "sensitive residues" in short-loop regions and mutated them to hydrophobic residues with large side chains to fill the cavities, thereby improving enzyme thermal stability. This strategy identified sensitive residues in the short-loop regions of three enzymes: lactate dehydrogenase from

Indexed as

biological sciencesbiotechnologyenzyme engineeringnatural sciences

Identifiers

PMID40212588

DOI10.1016/j.isci.2025.112202

PMCPMC11982487

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.