Histopathological observation and

Introduction: Wheat leaf rust is one of the most significant diseases affecting wheat. With the increase of global average temperature, the areas where wheat leaf rust can survive winter and persist through summer have been expanding. And high-temperature resistant isolates were identified within the natural population. Methods: In this study, two high-temperature resistant strains and two temperature-sensitive strains were selected from 41 isolates collected from Shanxi Province between 2021 and 2022. The analysis of disease severity differences among the four strains at different temperatures revealed that the differences were most significant at 26°C ( Results: Histopathological observations showed that compared with the temperature-sensitive strains, the number of haustorial mother cells and haustoria of high-temperature resistant was not significantly affected by high-temperature stress ( Discussion: However, the specific molecular mechanism in wheat leaf rust required further study and verification. In conclusion, the early germination of urediospores (0-12 h) and the formation of haustorial mother cells and haustoria (12-24 h) were key stages in resisting high-temperature stress in wheat leaf rust.