ArticleAnimal bioscience2026
Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits.
Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveThe ovary, as an important reproductive organ, tightly regulates the litter size of rabbits through its complex network of genes. This study aims to identify candidate genes related to litter size in rabbits through RNA sequencing and to analyze the regulatory effects of C-X-C motif chemokine ligand 12 (CXCL12) on granulosa cells (GCs).
methodsThis study used ovarian transcriptome sequencing to identify differentially expressed genes between the ovarian tissues of New Zealand female rabbits with high (H) and low (L) litter sizes. In addition, a new candidate gene which was highly expressed in group H, namely the chemokine ligand CXCL12, was selected for further verification of biological functions.
resultsThe cell counting kit-8 assay and flow cytometry analysis showed that CXCL12 can promote GCs proliferation but inhibit their apoptosis. Furthermore, quantitative reverse transcription polymerase chain reaction and western blotting indicated that CRABP1 regulated genes (PCNA, CCND1, CDK2, Bcl-2 and Bax) and proteins (CCND1, PCNA, Bcl-2 and Bax) related to cell cycle and cell proliferation. In addition, it can also regulate the expression levels of genes (TAF4B, CITED1, WNT2, WNT10B, and HSD17B1) and proteins (CITED1 and WNT10B) related to follicle development and litter size. Finally, it was found that CXCL12 targeted the CXCR4 receptor to activate the JAK/STAT signaling pathway.
conclusionWe utilized bioinformatics to screen 184 genes potentially associated with litter size in New Zealand female rabbits. Among these, CXCL12 plays a role in regulating the expression of cell cycle-related genes, promoting GCs proliferation. As a result, CXCL12 emerges as a promising candidate marker for selecting high litter size female rabbits in future breeding and production practices.
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