ArticleAnimal bioscience2025
CircEPDR1 regulates proliferation and differentiation of goat skeletal muscle satellite cells through miR-345-3p/Akirin1 axis.
Article in Animal bioscience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Knockdown of circFOXN3 Promotes the Proliferation and Differentiation of Bovine Myoblasts.Probiotics and antimicrobial proteins · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
objectiveCircular RNAs (circRNAs) play pivotal roles in regulating the growth and development of mammalian skeletal muscle This study aims to provide new insights into the molecu-lar regulatory network that underlies skeletal muscle development, presenting valuable targets to enhance and optimize livestock production performance.
methodsIn this study, we anchored a novel circRNA from previous circRNA sequencing data from goat skeletal muscle and named it circEPDR1, which was confirmed through en-zymatic digestion and Sanger sequencing. Functional investigation of circEPDR1 included overexpression experiments in goat muscle satellite cells (MuSCs), and its subcellular localization was determined using nuclear-cytoplasmic separation. Bioinformatics analysis was employed to reveal a circRNA-miRNA-RNA regulatory pathway involving circEPDR1. This process was further validated through Dual-luciferase reporter assay, real-time fluorescence quantitative polymerase chain reaction, Western blotting, EdU incorporation, CCK-8 cell viability tests, and immunofluorescence experiments.
resultsWe newly identified circEPDR1, which is produced by reverse splicing the second and third exons of the EPDR1 gene. circEPDR1 was enriched in skeletal muscle and dynamically expressed during the growth of goat MuSCs. In addition, circEPDR1 significantly inhibited the proliferation while promoting myogenic differentiation of goat MuSCs. The dominant cytoplasm distribution and enrichment by the AGO2 protein imply that circEPDR1 potentially works as a competitive endogenous RNA. Mechanically, miR-345-3p directly targeted circEPDR1, and its function in myogenesis was completely reversed to that of circEPDR1. Meanwhile, miR-345-3p negatively regulated Akirin1 mRNA through their base-pairing. Similar to circEPDR1, Akirin1 suppressed proliferation while also promoting differentiation.
conclusionThis study demonstrates that circEPDR1 controls a vital role in regulating the prolifera-tion and differentiation of goat MuSCs through the circEPDR1/miR-345-3p/Akirin1 axis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.