Evidence map›Paper›PMID 40211356›Full record

ArticlePlant methods2025

Citrus phloem specific transcriptional profiling through the development of a citrus tristeza virus expressed translating ribosome affinity purification system.

James N Culver, Meinhart Vallar, Erik Burchard, Sophie Kamens, Sebastien Lair, Yiping Qi, Tamara D Collum, Christopher Dardick, Choaa A El-Mohtar, Elizabeth E Rogers

Abstract read
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In one paragraph

Article in Plant methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

James N CulverInstitute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA. jculver@umd.edu.
Meinhart VallarInstitute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA.
Erik BurchardUSDA, Agricultural Research Service, Appalachian Fruit Research Station, Kearneysville, WV, USA.
Sophie KamensInstitute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA.
Sebastien LairInstitute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA.
Yiping QiDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
Tamara D CollumUSDA, Agricultural Research Service, Appalachian Fruit Research Station, Kearneysville, WV, USA.
Christopher DardickUSDA, Agricultural Research Service, Appalachian Fruit Research Station, Kearneysville, WV, USA.
Choaa A El-MohtarDepartment of Plant Pathology, Citrus Research and Education Center, University of Florida, Gainesville, FL, USA.
Elizabeth E RogersUSDA, Agricultural Research Service, Foreign Disease-Weed Science Research Unit, Frederick, MD, USA.

Funding

Citrus Research and Development Foundation, 18-019United States Department of Agriculture, National Institutes of Food and Agriculture 2020-70029-33161
6 · The paper itself

Abstract

backgroundThe analysis of translationally active mRNAs, or translatome, is a useful approach for monitoring cellular and plant physiological responses. One such method is the translating ribosome affinity purification (TRAP) system, which utilizes tagged ribosomal proteins to isolate ribosome-associated transcripts. This approach enables spatial and temporal gene expression analysis by driving the expression of tagged ribosomal proteins with tissue- or development-specific promoters. In plants, TRAP has enhanced our understanding of physiological responses to various biotic and abiotic factors. However, its utility is hampered by the necessity to generate transgenic plants expressing the tagged ribosomal protein, making this approach particularly challenging in perennial crops such as citrus.

resultsThis study involved the construction of a citrus tristeza virus (CTV) vector to express an immuno-tagged ribosome protein (CTV-hfRPL18). CTV, limited to the phloem, has been used for expressing marker and therapeutic sequences, making it suitable for analyzing citrus vascular tissue responses, including those related to huanglongbing disease. CTV-hfRPL18 successfully expressed a clementine-derived hfRPL18 peptide, and polysome purifications demonstrated enrichment for the hfRPL18 peptide. Subsequent translatome isolations from infected Nicotiana benthamiana and Citrus macrophylla showed enrichment for phloem-associated genes.

conclusionThe CTV-hfRPL18 vector offers a transgene-free and rapid system for TRAP expression and translatome analysis of phloem tissues within citrus.

Indexed as

Citrus translatome analysisPhloem gene expressionTranslating ribosome affinity purification (TRAP)Virus vector

Identifiers

PMID40211356
PMCPMC11983876

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.