Evidence map›Paper›PMID 40210952›Full record

ArticleScientific reports2025

A long-read sequencing approach to high-resolution profiling of bacterioplankton diversity in a shallow freshwater lake.

Stephanie O Castro Márquez, Viktor R Tóth, Sofiia Kolchanova, Walter W Wolfsberger, Taras K Oleksyk

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Stephanie O Castro MárquezDepartment of Biological Sciences, Oakland University, Rochester, MI, USA.ORCID http://orcid.org/0000-0002-9766-1299
Viktor R TóthHUN-REN Balaton Limnological Research Institute, Tihany, Hungary.ORCID http://orcid.org/0000-0002-6182-2759
Sofiia KolchanovaDepartment of Biology, University of Puerto Rico - Mayagüez, Mayagüez, Puerto Rico.ORCID http://orcid.org/0000-0002-1928-5486
Walter W WolfsbergerDepartment of Biological Sciences, Oakland University, Rochester, MI, USA.ORCID http://orcid.org/0000-0003-0980-645X
Taras K OleksykDepartment of Biological Sciences, Oakland University, Rochester, MI, USA. oleksyk@oakland.edu.ORCID http://orcid.org/0000-0002-8148-3918

Funding

Magyar Tudományos Akadémia RRF-2.3.1-21-2022-00008
6 · The paper itself

Abstract

Lake Balaton, a large shallow freshwater lake in Hungary, exhibits diverse bacterioplankton communities influenced by various environmental factors. This study aims to evaluate the bacterial diversity in Lake Balaton using the long-read approach to 16 S rRNA gene sequencing. Water samples were collected from a wide network of 33 locations across the lake's four basins and analyzed for bacterial community composition. Sequencing results revealed a high taxonomic diversity with significant zonal variations. Dominant families included Comamonadaceae, Burkholderiaceae, and Methylophilaceae. Environmental parameters such as temperature, pH, and CDOM were found to significantly correlate with bacterial abundance and diversity. The study underscores the utility and portability of using the long-read sequencing technology in assessing microbial diversity and provides insights into the ecological dynamics of bacterioplankton in freshwater lakes.

Indexed as

BacteriaBiodiversityLakesPlanktonFresh WaterHigh-Throughput Nucleotide SequencingHungaryPhylogenyRNA, Ribosomal, 16SSequence Analysis, DNAWater MicrobiologyRNA, Ribosomal, 16SLake BalatonLittoralLong read sequencingMicrobial communitiesNext generation sequencingOxford nanoporePelagic

Identifiers

PMID40210952
PMCPMC11986156

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.