Chronic wasting disease (CWD) prion detection in environmental and biological samples from a taxidermy site and nursing facility, and instruments used in surveillance activities.

Chronic wasting disease (CWD) is a transmissible prionopathy affecting free-ranging and captive cervids. CWD is thought to spread through both direct and indirect transmission mechanisms. Along this line, human activities have not been thoroughly explored for their potential to spread this disease. One area of concern involves taxidermy procedures and surveillance activities as handled animals or carcasses are of unknown CWD statuses. Worrisomely, taxidermy facilities can act as foci of prion infectivity if appropriate biosecurity practices are not implemented. In this study, we evaluated the presence of infectious prions in a taxidermy facility that was possibly exposed to CWD prions. To determine this, we collected biological and environmental specimens from this site and screened them using the protein misfolding cyclic amplification (PMCA) technique. Additionally, we swabbed different surfaces possibly exposed to CWD-infected animals or carcasses. We report the presence of prions in i) waters used to digest tissues from deer carcasses, ii) soils that were in contact with the previously mentioned waters, iii) dermestid beetles used to clean skulls, iv) other insects found in the beetle shed, and iv) dumpsters where animal carcasses were disposed. Additionally, we report that surgical materials used in surveillance practices may also hold CWD prions, even after being washed with aqueous solutions. All these results suggest that CWD prions may be disseminated due to human practices and that protocols should be established to decontaminate potentially contaminated materials.