Evidence map›Paper›PMID 40208540›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Methods for Assessing Scaffold Vascularization with Human Endothelial Cells.

Anne M Kong, Guei-Sheung Liu, Geraldine M Mitchell, Shiang Y Lim

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Anne M KongO'Brien Institute Department, St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia.
Guei-Sheung LiuOphthalmology, Department of Surgery, University of Melbourne, East Melbourne, VIC, Australia.
Geraldine M Mitchell *O'Brien Institute Department, St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia.
Shiang Y Lim *O'Brien Institute Department, St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia. mlim@svi.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The success of tissue engineering relies heavily on the efficient and rapid formation of blood vessels in the newly engineered tissues. To achieve this, it is crucial to use scaffolds that not only support the survival of vascular cells but also facilitate the development and maturation of a capillary network in vivo. Utilizing advanced biomaterials and scaffold designs is essential to ensure that the engineered tissues can integrate effectively with the host tissues and maintain their functionality over time. Here, we present a method for generating endothelial cells from induced pluripotent stem cells and pre-vascularizing scaffolds by seeding them with endothelial cells in a 3D porous structure. These pre-vascularized scaffolds can then be implanted in a rat subcutaneous model to create vascularized tissue constructs. This approach holds great promise for generating clinically viable constructs that can be used in regenerative medicine and drug development.

Indexed as

Endothelial CellsNeovascularization, PhysiologicTissue EngineeringTissue ScaffoldsAnimalsCell Culture TechniquesCell DifferentiationHumansInduced Pluripotent Stem CellsRatsEndothelial cellsExtrinsic vascularizationIntrinsic vascularizationScaffold vascularizationTissue engineering

Identifiers

PMID40208540

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.