Evidence map›Paper›PMID 40208538›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Characterization of Human Skin Derived Cells by Raman Micro-Spectroscopy.

Luca Pontiggia, Hesham Yosef, Thomas Biedermann, Ueli Moehrlen

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Luca PontiggiaTissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Zurich, Switzerland. luca.pontiggia@kispi.uzh.ch.ORCID 0000-0002-5553-8997
Hesham YosefmicrophotonX GmbH, Planegg, Germany.ORCID 0000-0002-2385-0046
Thomas BiedermannTissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Zurich, Switzerland.ORCID 0000-0002-6438-8791
Ueli MoehrlenTissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Zurich, Switzerland.ORCID 0000-0001-6418-1136

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studying human skin biology can aid in understanding the pathophysiology of skin diseases and developing novel therapies, including tissue engineering approaches. Among various optical and spectroscopic techniques, Raman micro-spectroscopy stands out. This non-invasive method requires minimal sample preparation and provides molecular-level information on healthy and diseased cell types. The following chapter aims to provide a comprehensive guide to the basic methods of Raman micro-spectroscopy analysis of human epidermal samples from the perspective of their biochemical-molecular relationship, without the need for a deep and specialized knowledge in the field of spectroscopy. We do this based on two distinct examples: (1) Unstained cell subpopulations identified by hierarchical cluster analysis are compared with antibody treated sorted cell populations, and (2) skin cell type-specific molecular features are determined, and the composition of unknown cellular mixtures is revealed.

Indexed as

SkinSpectrum Analysis, RamanCluster AnalysisHumansHierarchical cluster analysis of keratinocytesHuman skin cellsLinear discriminant analysis classification trainingPrincipal component analysisRaman micro-spectroscopy

Identifiers

PMID40208538

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.