ArticleMethods in molecular biology (Clifton, N.J.)2025
Deep Proteome Profiling of Primary Skin Fibroblasts.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- The nitrogen starvation-induced inhibitor Rts3 restrains Sit4/PP6 to gate quiescence downstream of TORC1.Nature communications · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Skin-derived primary cells and skin tissue are prime model systems to study molecular disease mechanisms on cell and tissue level. Cells and tissue can be cultivated ex vivo and protocols for both, simple 2D as well as 3D in vitro cultures reflecting different levels of complexity exist. Mass spectrometry (MS)-based proteomics is a prime approach to link molecular mechanisms to observable disease phenotypes. In this chapter, we describe in detail the analysis of 2D and 3D primary skin fibroblast cultures by MS. We focus on automated sample processing to increase throughput and usage of limited cell numbers to reduce costs. The described workflow supports the study of proteome regulation in large scale screening approaches, be it for drug discovery or in clinical studies.
Indexed as
Identifiers
40208537What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.