Engineering a Multilayered Skin Equivalent: The Importance of Endogenous Extracellular Matrix and Model Flexibility for Nuanced and Accurate Skin Studies.

Lucy Smith, Victoria Maltman, Paola De Los Santos Gomez, Kirsty Goncalves, Stefan Przyborski

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lucy SmithDepartment of Biosciences, Durham University, Durham, UK.

Victoria MaltmanDepartment of Biosciences, Durham University, Durham, UK.

Paola De Los Santos GomezDepartment of Biosciences, Durham University, Durham, UK.

Kirsty GoncalvesDepartment of Biosciences, Durham University, Durham, UK.

Stefan PrzyborskiDepartment of Biosciences, Durham University, Durham, UK. stefan.przyborski@durham.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human skin equivalents (HSEs) are a valuable tool for both academic and industrial laboratories to further the understanding of skin physiology in a range of health and disease situations. There have been many advances in the development of HSEs that successfully recapitulate the structure of human skin in vitro; however a main limitation is variability due to the use of complex protocols and exogenous extracellular matrix (ECM) proteins. Previously, we have developed a robust, reproducible, and unique full thickness HSE, using a consistent scaffold, commercially available cells and defined low-serum media. Alvetex

Indexed as

Extracellular MatrixSkinSkin, ArtificialTissue EngineeringCell Culture TechniquesCell DifferentiationCells, CulturedExtracellular Matrix ProteinsFibroblastsHumansKeratinocytesTissue ScaffoldsExtracellular Matrix ProteinsAlvetex® scaffoldClinical measuresComplex modelsEndogenous extracellular matrix depositionOrganotypic cultureSkin equivalentSkin tissue engineering

Identifiers

PMID40208534

DOI10.1016/j.jaad.2024.01.067

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.