Evidence map›Paper›PMID 40208529›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Isolation and Culture of Human Dermal Microvascular Endothelial Cells.

Jennifer Bourland, Dominique Mayrand, Nathalie Tremblay, Véronique J Moulin, Julie Fradette, François A Auger

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jennifer BourlandCentre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Québec, QC, Canada.
Dominique MayrandCentre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Québec, QC, Canada.
Nathalie TremblayCentre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Québec, QC, Canada.
Véronique J MoulinCentre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Québec, QC, Canada. veronique.moulin@fmed.ulaval.ca.
Julie FradetteCentre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Québec, QC, Canada. Julie.fradette@fmed.ulaval.ca.
François A AugerCentre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Québec, QC, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Primary endothelial cells are needed for angiogenesis studies, and more particularly in the field of tissue engineering, to generate pre-vascularized tissues in vitro. Investigations often use human umbilical vein endothelial cells due to their extensive characterization, but also because they are easy to obtain and isolate. An alternative is the use of human dermal microvascular endothelial cells, more representative of adult skin angiogenic processes. This chapter presents a detailed methodology to isolate and culture microvascular endothelial cells from human skin biopsies based on enzymatic digestion and mechanical extraction.

Indexed as

Cell Culture TechniquesCell SeparationDermisEndothelial CellsMicrovesselsSkinCells, CulturedHumansAngiogenesisCapillaryCell isolationEndothelial cellsImmunoselectionMicrovascularizationSkinTissue engineering

Identifiers

PMID40208529

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.