Antimicrobial applications of inorganic radiosensitizers and their potential in biofilm control.

Biofilms are structured microbial communities embedded in a self-produced extracellular matrix. This lifestyle provides significant protection against environmental stressors such as desiccation, chemical treatments and even ionizing radiation. Radiation, while a well-established antibacterial strategy, can be less effective in biofilms. Biofilm superior resilience is due to several advantages such as the shielding provided by the matrix, the metabolic heterogeneity and adaptive stress responses of biofilm-associated cells. To address this challenge, researchers are increasingly employing combination strategies in antibiofilm treatment. Radiosensitizers, compounds originally developed to enhance the efficacy of radiation therapy in cancer treatment, have also garnered attention for their potential in antimicrobial applications. These compounds act by amplifying the effects of radiation, often through mechanisms such as increased oxidative stress or inhibition of DNA repair pathways. However, research on radiosensitizers in bacterial systems has focused on planktonic cultures, with limited studies exploring their effects on biofilms. Given the complexity and unique characteristics of biofilms, their response to radiosensitization remains poorly understood and requires further investigation. The use of radiosensitizers in conjunction with radiation presents a promising approach to overcome the inherent resilience of biofilms. By enhancing the susceptibility of biofilm-associated bacteria to radiation and simultaneously disrupting their protective structures, such approaches could lead to more effective and comprehensive solutions. Understanding the nuanced responses of biofilms to these combined treatments is essential for advancing both medical and environmental applications and addressing the challenge of biofilm persistence.