ArticleCurrent microbiology2025
Cloning and Expression of the Isoprocarb Hydrolase Gene cehA from the Newly Isolated Isoprocarb-Degrading Strain Sphingobium sp. R-7.
Article in Current microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- The PucR-type activator IppR regulates 2-isopropylphenol hydroxylation in strainApplied and environmental microbiology · 2026Article
- Identification of a novelApplied and environmental microbiology · 2025Article
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Authors and funding
6 authors.
Funding
Abstract
To remove the isoprocarb residues from the environment, a bacterial strain that was capable of degrading isoprocarb was isolated from agricultural soils where isoprocarb has been applied for a long period, and named R-7. On the basis of its cellular morphology and phenotypic features and 16S rRNA phylogenetic analysis, strain R-7 was preliminarily identified as Sphingobium sp. This strain could grow on isoprocarb as a sole carbon source and degrade 98.3% of 0.5 mM of isoprocarb within 16 h in batch liquid culture. The metabolite produced was identified as 2-isopropylphenol by high-performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS) analysis. Strain R-7 hydrolysed the ester bond of isoprocarb to generate 2-isopropylphenol, but this metabolite was not further degraded. Upon the genome comparison, the isoprocarb hydrolase gene cehA was cloned from strain R-7 and expressed in Escherichia coli BL21. The purified CehA
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